DNA ligation using a disposable microfluidic device combined with a micromixer and microchannel reactor
DC Field | Value | Language |
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dc.contributor.author | Ko, Yong-Jun | - |
dc.contributor.author | Maeng, Joon-Ho | - |
dc.contributor.author | Ahn, Yoomin | - |
dc.contributor.author | Hwang, Seung Yong | - |
dc.date.accessioned | 2021-06-23T10:06:49Z | - |
dc.date.available | 2021-06-23T10:06:49Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2011-10 | - |
dc.identifier.issn | 0925-4005 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/36516 | - |
dc.description.abstract | A novel PDMS and glass-based microfluidic device consisting of a micromixer and microreactor for DNA ligation is described in this article. The new passive type planar micromixer is 10.33 mm long and composed of a straight channel integrated with nozzles and pillars, and the microreactor is composed of a serpentine channel. Mixing was enhanced by convective diffusion facilitated by the nozzles and pillars. The performance of the micromixer was analytically simulated and experimentally evaluated. The micromixer showed a good mixing efficiency of 87.7% at a 500 mu L/min flow rate (Re = 66.5). DNA ligation was successfully performed using the new microfluidic device, and ligation time was shortened from 4 h to 5 min. When used for on-chip ligation, this new micromixer offers advantages of disposability and portability. (C) 2011 Elsevier B.V. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Elsevier BV | - |
dc.title | DNA ligation using a disposable microfluidic device combined with a micromixer and microchannel reactor | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Ahn, Yoomin | - |
dc.contributor.affiliatedAuthor | Hwang, Seung Yong | - |
dc.identifier.doi | 10.1016/j.snb.2011.05.016 | - |
dc.identifier.scopusid | 2-s2.0-79959677419 | - |
dc.identifier.wosid | 000293161300058 | - |
dc.identifier.bibliographicCitation | Sensors and Actuators, B: Chemical, v.157, no.2, pp.735 - 741 | - |
dc.relation.isPartOf | Sensors and Actuators, B: Chemical | - |
dc.citation.title | Sensors and Actuators, B: Chemical | - |
dc.citation.volume | 157 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 735 | - |
dc.citation.endPage | 741 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalResearchArea | Instruments & Instrumentation | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Instruments & Instrumentation | - |
dc.subject.keywordPlus | MIXER | - |
dc.subject.keywordPlus | PCR | - |
dc.subject.keywordAuthor | DNA ligation | - |
dc.subject.keywordAuthor | Micromixer | - |
dc.subject.keywordAuthor | Micropillar | - |
dc.subject.keywordAuthor | Micronozzle | - |
dc.subject.keywordAuthor | Serpentine microreactor | - |
dc.subject.keywordAuthor | PDMS-glass microbiochip | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0925400511004175?via%3Dihub | - |
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