Loop-Mediated Isothermal Amplification Assay for Detection of Haemophilus influenzae Type b in Cerebrospinal Fluid
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Dong Wook | - |
dc.contributor.author | Kilgore, Paul Evan | - |
dc.contributor.author | Kim, Eun Jin | - |
dc.contributor.author | Kim, Soon Ae | - |
dc.contributor.author | Dang Duc Anh | - |
dc.contributor.author | Seki, Mitsuko | - |
dc.date.accessioned | 2021-06-23T10:37:06Z | - |
dc.date.available | 2021-06-23T10:37:06Z | - |
dc.date.issued | 2011-10 | - |
dc.identifier.issn | 0095-1137 | - |
dc.identifier.issn | 1098-660X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/37171 | - |
dc.description.abstract | Haemophilus influenzae type b (Hib) is one of the leading causes of meningitis in developing countries. To establish and evaluate a novel loop-mediated isothermal amplification (LAMP) assay for Hib, we designed a LAMP primer set targeting the Hib-specific capsulation locus. LAMP detected 10 copies of purified DNA in a 60-min reaction. This indicated that the detection limit of LAMP was > 100-fold lower than the detection limits of both a PCR for the detection of bexA and a nested PCR for Hib (Hib PCR). No H. influenzae, other than Hib or control bacteria, was detected. Linear determination ranged from 10 to 1,000,000 microorganisms per reaction mixture using real-time turbidimetry. We evaluated the Hib LAMP assay using a set of 52 randomly selected cerebrospinal fluid (CSF) specimens obtained from children with suspected meningitis. For comparison, the CSF specimens were tested using a conventional Hib PCR assay. Hib was detected in 30 samples using LAMP and in 22 samples using the Hib PCR assay. The Hib PCR showed a clinical sensitivity of 73.3% and a clinical specificity of 100% relative to the Hib LAMP assay. These results suggest that further development and evaluation of the Hib LAMP will enhance the global diagnostic capability for Hib detection. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | American Society for Microbiology | - |
dc.title | Loop-Mediated Isothermal Amplification Assay for Detection of Haemophilus influenzae Type b in Cerebrospinal Fluid | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1128/JCM.00515-11 | - |
dc.identifier.scopusid | 2-s2.0-80053547491 | - |
dc.identifier.wosid | 000295360700029 | - |
dc.identifier.bibliographicCitation | Journal of Clinical Microbiology, v.49, no.10, pp 3621 - 3626 | - |
dc.citation.title | Journal of Clinical Microbiology | - |
dc.citation.volume | 49 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 3621 | - |
dc.citation.endPage | 3626 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | REAL-TIME PCR | - |
dc.subject.keywordPlus | STREPTOCOCCUS-PNEUMONIAE | - |
dc.subject.keywordPlus | BACTERIAL-MENINGITIS | - |
dc.subject.keywordPlus | DISEASE BURDEN | - |
dc.subject.keywordPlus | VACCINE | - |
dc.subject.keywordPlus | LAMP | - |
dc.subject.keywordPlus | CHILDREN | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordAuthor | REAL-TIME PCR | - |
dc.subject.keywordAuthor | STREPTOCOCCUS-PNEUMONIAE | - |
dc.subject.keywordAuthor | BACTERIAL-MENINGITIS | - |
dc.subject.keywordAuthor | GENE | - |
dc.subject.keywordAuthor | DNA | - |
dc.subject.keywordAuthor | DISEASE BURDEN | - |
dc.subject.keywordAuthor | VACCINE | - |
dc.subject.keywordAuthor | LAMP | - |
dc.subject.keywordAuthor | CHILDREN | - |
dc.identifier.url | https://journals.asm.org/doi/10.1128/JCM.00515-11 | - |
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