In vitro expansion of human adipose-derived stem cells in a spinner culture system using human extracellular matrix powders
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Ji Suk | - |
dc.contributor.author | Kim, Beob Soo | - |
dc.contributor.author | Kim, Jae Dong | - |
dc.contributor.author | Choi, Young Chan | - |
dc.contributor.author | Lee, Eun Kyu | - |
dc.contributor.author | Park, Kinam | - |
dc.contributor.author | Lee, Hee Young | - |
dc.contributor.author | Cho, Yong Woo | - |
dc.date.accessioned | 2021-06-23T10:38:25Z | - |
dc.date.available | 2021-06-23T10:38:25Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2011-09 | - |
dc.identifier.issn | 0302-766X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/37220 | - |
dc.description.abstract | Stem cell therapy requires large numbers of stem cells to replace damaged tissues, but only limited numbers of stem cells can be harvested from a single patient. To obtain large quantities of stem cells with differentiation potential, we explored a spinner culture system using human extracellular matrix (hECM) powders. The hECM was extracted from adipose tissue and fabricated into powders. Human adipose-derived stem cells (hASCs) were isolated, seeded on hECM powders, and cultivated in a spinner flask. The 3-D culture system, using hECM powders, was highly effective for promoting cell proliferation. The number of hASCs in the 3-D culture system significantly increased for 10 days, resulting in an approximately 10-fold expansion, whereas a traditional 2-D culture system showed just a 2.8-fold expansion. Surface markers, transcriptional factors, and differentiation potential of hASCs were assayed to identify the characteristics of proliferated cells in 3-D culture system. The hASCs expressed the pluripotency markers, Oct-4 and Sox-2 during 3-D culture and retained their capacity to differentiate into adipogenic, osteogenic, and chondrogenic lineages. These findings demonstrate that the 3-D culture systems using hECM powders provide an efficient in vitro environment for stem cell proliferation, and could act as stem cell delivery carriers for autologous tissue engineering and cell therapy. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER | - |
dc.title | In vitro expansion of human adipose-derived stem cells in a spinner culture system using human extracellular matrix powders | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Cho, Yong Woo | - |
dc.identifier.doi | 10.1007/s00441-011-1223-5 | - |
dc.identifier.scopusid | 2-s2.0-80052764315 | - |
dc.identifier.wosid | 000294681100011 | - |
dc.identifier.bibliographicCitation | CELL AND TISSUE RESEARCH, v.345, no.3, pp.415 - 423 | - |
dc.relation.isPartOf | CELL AND TISSUE RESEARCH | - |
dc.citation.title | CELL AND TISSUE RESEARCH | - |
dc.citation.volume | 345 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 415 | - |
dc.citation.endPage | 423 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | TISSUE | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | MICROCARRIERS | - |
dc.subject.keywordPlus | MARROW | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | PROGENITORS | - |
dc.subject.keywordPlus | THERAPIES | - |
dc.subject.keywordPlus | VIVO | - |
dc.subject.keywordAuthor | Adipose-derived stem cell | - |
dc.subject.keywordAuthor | hECM powders | - |
dc.subject.keywordAuthor | 3-D culture system | - |
dc.subject.keywordAuthor | Large expansion | - |
dc.subject.keywordAuthor | Autologous tissue engineering | - |
dc.subject.keywordAuthor | Human | - |
dc.identifier.url | https://link.springer.com/article/10.1007%2Fs00441-011-1223-5 | - |
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