Log-scale dose response of inhibitors on a chip
DC Field | Value | Language |
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dc.contributor.author | Yun, Jae-young | - |
dc.contributor.author | Jambovane, Sachin R. | - |
dc.contributor.author | Kim, Se-kwon | - |
dc.contributor.author | Cho, Sung-hak | - |
dc.contributor.author | Duin, Evert C. | - |
dc.contributor.author | Hong, Jong Wook | - |
dc.date.accessioned | 2021-06-23T10:39:03Z | - |
dc.date.available | 2021-06-23T10:39:03Z | - |
dc.date.created | 2021-02-18 | - |
dc.date.issued | 2011-08 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/37247 | - |
dc.description.abstract | We demonstrate the accommodation of log-scale concentration gradients of inhibitors on a single microfluidic chip with a semidirect dilution capability of reagents for the determination of the half-inhibitory concentration or IC 50. The chip provides a unique tool for hosting a wide-range of concentration gradient for studies that require an equal distribution of measuring points on a logarithmic scale. Using Matrix metalloproteinase IX and three of its inhibitors, marimastat, batimastat, and CP471474, we evaluated the IC 50 of each inhibitor with a single experiment. The present work could be applied to the systematic study of biochemical binding and inhibition processes particularly in the field of mechanistic enzymology and the pharmaceutical industry. © 2011 American Chemical Society. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Log-scale dose response of inhibitors on a chip | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Hong, Jong Wook | - |
dc.identifier.doi | 10.1021/ac201177g | - |
dc.identifier.scopusid | 2-s2.0-80051770343 | - |
dc.identifier.wosid | 000293758800006 | - |
dc.identifier.bibliographicCitation | ANALYTICAL CHEMISTRY, v.83, no.16, pp.6148 - 6153 | - |
dc.relation.isPartOf | ANALYTICAL CHEMISTRY | - |
dc.citation.title | ANALYTICAL CHEMISTRY | - |
dc.citation.volume | 83 | - |
dc.citation.number | 16 | - |
dc.citation.startPage | 6148 | - |
dc.citation.endPage | 6153 | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | MATRIX-METALLOPROTEINASE INHIBITORS | - |
dc.subject.keywordPlus | MICROFLUIDIC DEVICE | - |
dc.subject.keywordPlus | KINETIC-PARAMETERS | - |
dc.subject.keywordPlus | ENZYME ASSAYS | - |
dc.subject.keywordPlus | GENERATION | - |
dc.subject.keywordPlus | MICROCHIP | - |
dc.subject.keywordPlus | GRADIENT | - |
dc.subject.keywordPlus | READERCELLS | - |
dc.identifier.url | https://pubs.acs.org/doi/10.1021/ac201177g | - |
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