A Tcf/Lef element within the enhancer region of the human NANOG gene plays a role in promoter activation
DC Field | Value | Language |
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dc.contributor.author | Kim, Chang Gun | - |
dc.contributor.author | Chung, Il-Yup | - |
dc.contributor.author | Lim, Yoongho | - |
dc.contributor.author | Lee, Young Han | - |
dc.contributor.author | Shin, Soon Young | - |
dc.date.accessioned | 2021-06-23T10:40:05Z | - |
dc.date.available | 2021-06-23T10:40:05Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2011-07 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/37293 | - |
dc.description.abstract | NANOG is a homeodomain-containing transcription factor that is essential for the maintenance of pluripotency and self-renewal in embryonic stem cells. However, the molecular mechanisms underlying the regulation of NANOG expression in human cells remain largely unknown. Here, we investigated the role of Tcf/Lef response elements located in the enhancer of the human NANOG gene. We found that forced expression of Lef1 or beta-catenin stimulated human NANOG promoter activity, while shRNA-mediated knockdown of beta-catenin reduced Lef1-induced NANOG promoter activation. Deletion or mutation of the Tcf/Lef element within the enhancer region of the human NANOG gene completely abrogated Lef1-induced NANOG promoter activity. The results of a chromatin immunoprecipitation assay demonstrated that Lefl and beta-catenin bind to the Tcf/Lef element in the enhancer region of the NANOG gene. Forced expression of GSK-31 inhibited basal, Lefl -induced, and beta-catenin-induced NANOG promoter activity, while treatment with the GSK-3 beta inhibitor SB216763 resulted in the accumulation of beta-catenin and NANOG protein. Furthermore, DvI-1-induced NANOG promoter activity was abrogated by the expression of beta-catenin shRNA. Stable overexpression of Dvl-1 caused beta-catenin and NANOG to accumulate. These results indicate that the Tcf/Lef response element in the enhancer region of the human NANOG gene is able to stimulate NANOG gene transcription. (C) 2011 Elsevier Inc. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Academic Press | - |
dc.title | A Tcf/Lef element within the enhancer region of the human NANOG gene plays a role in promoter activation | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chung, Il-Yup | - |
dc.identifier.doi | 10.1016/j.bbrc.2011.06.044 | - |
dc.identifier.scopusid | 2-s2.0-79960034683 | - |
dc.identifier.wosid | 000292797700046 | - |
dc.identifier.bibliographicCitation | Biochemical and Biophysical Research Communications, v.410, no.3, pp.637 - 642 | - |
dc.relation.isPartOf | Biochemical and Biophysical Research Communications | - |
dc.citation.title | Biochemical and Biophysical Research Communications | - |
dc.citation.volume | 410 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 637 | - |
dc.citation.endPage | 642 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.subject.keywordPlus | EMBRYONIC STEM-CELLS | - |
dc.subject.keywordPlus | ES CELLS | - |
dc.subject.keywordPlus | SELF-RENEWAL | - |
dc.subject.keywordPlus | CYCLIN D1 | - |
dc.subject.keywordPlus | PLURIPOTENCY | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | WNT | - |
dc.subject.keywordPlus | TARGET | - |
dc.subject.keywordAuthor | NANOG | - |
dc.subject.keywordAuthor | Dvl-1 | - |
dc.subject.keywordAuthor | Tcf/Lef | - |
dc.subject.keywordAuthor | beta-Catenin | - |
dc.subject.keywordAuthor | GSK-3 beta | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0006291X11010023?via%3Dihub | - |
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