Identification of Chromatophore Membrane Protein Complexes Formed under Different Nitrogen Availability Conditions in Rhodospirillum rubrum
DC Field | Value | Language |
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dc.contributor.author | Selao, Tiago Toscano | - |
dc.contributor.author | Branca, Rui | - |
dc.contributor.author | Chae, Pil Seok | - |
dc.contributor.author | Lehtio, Janne | - |
dc.contributor.author | Gellman, Samuel H. | - |
dc.contributor.author | Rasmussen, Soren G. F. | - |
dc.contributor.author | Nordlund, Stefan | - |
dc.contributor.author | Noren, Agneta | - |
dc.date.accessioned | 2021-06-23T10:41:49Z | - |
dc.date.available | 2021-06-23T10:41:49Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2011-06 | - |
dc.identifier.issn | 1535-3893 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/37406 | - |
dc.description.abstract | The chromatophore membrane of the photosynthetic diazotroph Rhodospirillum rubrum is of vital importance for a number of central processes, including nitrogen fixation. Using a novel amphiphile, we have identified protein complexes present under different nitrogen availability conditions by the use of two-dimensional Blue Native/SDS-PAGE and NSI-LC-LTQ-Orbitrap mass spectrometry. We have identified several membrane protein complexes, including components of the ATP synthase, reaction center, light harvesting, and NADH dehydrogenase complexes. Additionally, we have identified differentially expressed proteins, such as subunits of the succinate dehydrogenase complex and other TCA cycle enzymes that are usually found in the cytosol, thus hinting at a possible association to the membrane in response to nitrogen deficiency. We propose a redox sensing mechanism that can influence the membrane subproteome in response to nitrogen availability. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | American Chemical Society | - |
dc.title | Identification of Chromatophore Membrane Protein Complexes Formed under Different Nitrogen Availability Conditions in Rhodospirillum rubrum | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chae, Pil Seok | - |
dc.identifier.doi | 10.1021/pr100838x | - |
dc.identifier.scopusid | 2-s2.0-79958199290 | - |
dc.identifier.wosid | 000291186100002 | - |
dc.identifier.bibliographicCitation | Journal of Proteome Research, v.10, no.6, pp.2703 - 2714 | - |
dc.relation.isPartOf | Journal of Proteome Research | - |
dc.citation.title | Journal of Proteome Research | - |
dc.citation.volume | 10 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 2703 | - |
dc.citation.endPage | 2714 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.subject.keywordPlus | LIGHT-HARVESTING COMPLEX | - |
dc.subject.keywordPlus | ATOMIC-FORCE MICROSCOPY | - |
dc.subject.keywordPlus | ACID CYCLE ENZYMES | - |
dc.subject.keywordPlus | RHODOBACTER-SPHAEROIDES | - |
dc.subject.keywordPlus | ELECTRON-TRANSPORT | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | OUTER-MEMBRANE | - |
dc.subject.keywordPlus | PHOTOSYNTHETIC MEMBRANE | - |
dc.subject.keywordPlus | ATP SYNTHASE | - |
dc.subject.keywordPlus | BACTERIAL CHROMATOPHORES | - |
dc.subject.keywordAuthor | Rhodospirillum rubrum | - |
dc.subject.keywordAuthor | nitrogen metabolism | - |
dc.subject.keywordAuthor | Blue Native | - |
dc.subject.keywordAuthor | chromatophore subproteome | - |
dc.subject.keywordAuthor | amphiphile | - |
dc.subject.keywordAuthor | Orbitrap | - |
dc.identifier.url | https://pubs.acs.org/doi/10.1021/pr100838x | - |
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