Enzyme Kinetic Measurements Using a Droplet-Based Microfluidic System with a Concentration Gradient
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Bui, Minh-Phuong Ngoc | - |
dc.contributor.author | Li, Cheng Ai | - |
dc.contributor.author | Han, Kwi Nam | - |
dc.contributor.author | Choo, Jaebum | - |
dc.contributor.author | Lee, Eun Kyu | - |
dc.contributor.author | Seong, Gi Hun | - |
dc.date.accessioned | 2021-06-23T11:04:40Z | - |
dc.date.available | 2021-06-23T11:04:40Z | - |
dc.date.issued | 2011-03 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.issn | 1520-6882 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/38210 | - |
dc.description.abstract | In this Paper We propose a microfluidic device that is capable Of generating a concentration gradient followed by parallel droplet formation within channels with a simple T-junction geometry. Linear concentration gradient profiles can be obtained based on fluid diffusion under laminar flow. Optimized conditions for generating a linear concentration gradient and parallel droplet formation were investigated using fluorescent dye. The concentration gradient profile under diffusive Mixing was dominated by the flow rate at sample inlets, while parallel droplet formation was affected by the channel geometry at both the inlet and outlet The microfluidic device was experimentally characterized using optimal layout and operating conditions,selected through a design process. Furthermore, in situ enzyme kinetic measurements of the beta- galactosidase-catalyzed hydrolysis of resorufin-beta-D-galactopyranoside were,performed to demonstrate the application potential of our Simple, time-effective, and low sample volume microfluidic device. We expect that, in addition to enzyme kinetics, drug screening and clinical diagnostic tests can be rapidly and accurately performed using this droplet-based microfluidic system. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Enzyme Kinetic Measurements Using a Droplet-Based Microfluidic System with a Concentration Gradient | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1021/ac102472a | - |
dc.identifier.scopusid | 2-s2.0-79952147403 | - |
dc.identifier.wosid | 000287685800018 | - |
dc.identifier.bibliographicCitation | ANALYTICAL CHEMISTRY, v.83, no.5, pp 1603 - 1608 | - |
dc.citation.title | ANALYTICAL CHEMISTRY | - |
dc.citation.volume | 83 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 1603 | - |
dc.citation.endPage | 1608 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | NANOLITER PLUGS | - |
dc.subject.keywordPlus | LAMINAR-FLOW | - |
dc.subject.keywordPlus | DEVICE | - |
dc.subject.keywordPlus | GENERATION | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordPlus | CHIP | - |
dc.subject.keywordPlus | CRYSTALLIZATION | - |
dc.subject.keywordPlus | OPTIMIZATION | - |
dc.subject.keywordPlus | PARAMETERS | - |
dc.identifier.url | https://pubs.acs.org/doi/10.1021/ac102472a | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
55 Hanyangdeahak-ro, Sangnok-gu, Ansan, Gyeonggi-do, 15588, Korea+82-31-400-4269 sweetbrain@hanyang.ac.kr
COPYRIGHT © 2021 HANYANG UNIVERSITY. ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.