The Crucial Role of GATA-1 in CCR3 Gene Transcription: Modulated Balance by Multiple GATA Elements in the CCR3 Regulatory Region
DC Field | Value | Language |
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dc.contributor.author | Kim, Byung Soo | - |
dc.contributor.author | Uhm, Tae Gi | - |
dc.contributor.author | Lee, Seol Kyoung | - |
dc.contributor.author | Lee, Sin-Hwa | - |
dc.contributor.author | Kang, Jin Hyun | - |
dc.contributor.author | Park, Choon-Sik | - |
dc.contributor.author | Chung, Il Yup | - |
dc.date.accessioned | 2021-06-23T12:36:55Z | - |
dc.date.available | 2021-06-23T12:36:55Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2010-12 | - |
dc.identifier.issn | 0022-1767 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/39297 | - |
dc.description.abstract | GATA-1, a zinc finger-containing transcription factor, regulates not only the differentiation of eosinophils but also the expression of many eosinophil-specific genes. In the current study, we dissected CCR3 gene expression at the molecular level using several cell types that express varying levels of GATA-1 and CCR3. Chromatin immunoprecipitation analysis revealed that GATA-1 preferentially bound to sequences in both exon 1 and its proximal intron 1. A reporter plasmid assay showed that constructs harboring exon 1 and/or intron 1 sequences retained transactivation activity, which was essentially proportional to cellular levels of endogenous GATA-1. Introduction of a dominant-negative GATA-1 or small interfering RNA of GATA-1 resulted in a decrease in transcription activity of the CCR3 reporter. Both point mutation and EMSA analyses demonstrated that although GATA-1 bound to virtually all seven putative GATA elements present in exon 1-intron 1, the first GATA site in exon 1 exhibited the highest binding affinity for GATA-1 and was solely responsible for GATA-1-mediated transactivation. The fourth and fifth GATA sites in exon 1, which were postulated previously to be a canonical double-GATA site for GATA-1-mediated transcription of eosinophil-specific genes, appeared to play an inhibitory role in transactivation, albeit with a high affinity for GATA-1. Furthermore, mutation of the seventh GATA site ( present in intron 1) increased transcription, suggesting an inhibitory role. These data suggest that GATA-1 controls CCR3 transcription by interacting dynamically with the multiple GATA sites in the regulatory region of the CCR3 gene. The Journal of Immunology, 2010, 185: 6866-6875. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | American Association of Immunologists | - |
dc.title | The Crucial Role of GATA-1 in CCR3 Gene Transcription: Modulated Balance by Multiple GATA Elements in the CCR3 Regulatory Region | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chung, Il Yup | - |
dc.identifier.doi | 10.4049/jimmunol.1001037 | - |
dc.identifier.scopusid | 2-s2.0-78650654759 | - |
dc.identifier.wosid | 000284311500053 | - |
dc.identifier.bibliographicCitation | Journal of Immunology, v.185, no.11, pp.6866 - 6875 | - |
dc.relation.isPartOf | Journal of Immunology | - |
dc.citation.title | Journal of Immunology | - |
dc.citation.volume | 185 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 6866 | - |
dc.citation.endPage | 6875 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.subject.keywordPlus | MAJOR BASIC-PROTEIN | - |
dc.subject.keywordPlus | CHEMOKINE RECEPTOR-3 | - |
dc.subject.keywordPlus | DNA-BINDING | - |
dc.subject.keywordPlus | AIRWAY INFLAMMATION | - |
dc.subject.keywordPlus | TISSUE EOSINOPHILIA | - |
dc.subject.keywordPlus | EPITHELIAL-CELLS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PROMOTER | - |
dc.subject.keywordPlus | EOTAXIN | - |
dc.subject.keywordPlus | ASTHMA | - |
dc.identifier.url | https://www.jimmunol.org/content/185/11/6866 | - |
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