Maltose-neopentyl glycol (MNG) amphiphiles for solubilization, stabilization and crystallization of membrane proteins
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chae, Pil Seok | - |
dc.contributor.author | Rasmussen, Soren G. F. | - |
dc.contributor.author | Rana, Rohini R. | - |
dc.contributor.author | Gotfryd, Kamil | - |
dc.contributor.author | Chandra, Richa | - |
dc.contributor.author | Goren, Michael A. | - |
dc.contributor.author | Kruse, Andrew C. | - |
dc.contributor.author | Nurva, Shailika | - |
dc.contributor.author | Loland, Claus J. | - |
dc.contributor.author | Pierre, Yves | - |
dc.contributor.author | Drew, David | - |
dc.contributor.author | Popot, Jean-Luc | - |
dc.contributor.author | Picot, Daniel | - |
dc.contributor.author | Fox, Brian G. | - |
dc.contributor.author | Guan, Lan | - |
dc.contributor.author | Gether, Ulrik | - |
dc.contributor.author | Byrne, Bernadette | - |
dc.contributor.author | Kobilka, Brian | - |
dc.contributor.author | Gellman, Samuel H. | - |
dc.date.accessioned | 2021-06-23T12:37:10Z | - |
dc.date.available | 2021-06-23T12:37:10Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2010-12 | - |
dc.identifier.issn | 1548-7091 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/39316 | - |
dc.description.abstract | The understanding of integral membrane protein (IMP) structure and function is hampered by the difficulty of handling these proteins. Aqueous solubilization, necessary for many types of biophysical analysis, generally requires a detergent to shield the large lipophilic surfaces of native IMPs. Many proteins remain difficult to study owing to a lack of suitable detergents. We introduce a class of amphiphiles, each built around a central quaternary carbon atom derived from neopentyl glycol, with hydrophilic groups derived from maltose. Representatives of this maltose-neopentyl glycol (MNG) amphiphile family show favorable behavior relative to conventional detergents, as manifested in multiple membrane protein systems, leading to enhanced structural stability and successful crystallization. MNG amphiphiles are promising tools for membrane protein science because of the ease with which they may be prepared and the facility with which their structures may be varied. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Nature Publishing Group | - |
dc.title | Maltose-neopentyl glycol (MNG) amphiphiles for solubilization, stabilization and crystallization of membrane proteins | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chae, Pil Seok | - |
dc.identifier.doi | 10.1038/NMETH.1526 | - |
dc.identifier.scopusid | 2-s2.0-78649693871 | - |
dc.identifier.wosid | 000284686300016 | - |
dc.identifier.bibliographicCitation | Nature Methods, v.7, no.12, pp.1003 - 1008 | - |
dc.relation.isPartOf | Nature Methods | - |
dc.citation.title | Nature Methods | - |
dc.citation.volume | 7 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 1003 | - |
dc.citation.endPage | 1008 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.subject.keywordPlus | MELIBIOSE PERMEASE | - |
dc.subject.keywordPlus | CRYSTAL-STRUCTURE | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | SURFACTANTS | - |
dc.subject.keywordPlus | OVEREXPRESSION | - |
dc.subject.keywordPlus | OPTIMIZATION | - |
dc.subject.keywordPlus | DETERGENTS | - |
dc.subject.keywordPlus | NANODISCS | - |
dc.subject.keywordPlus | AMPHIPOLS | - |
dc.identifier.url | https://www.nature.com/articles/nmeth.1526 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
55 Hanyangdeahak-ro, Sangnok-gu, Ansan, Gyeonggi-do, 15588, Korea+82-31-400-4269 sweetbrain@hanyang.ac.kr
COPYRIGHT © 2021 HANYANG UNIVERSITY. ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.