The phosphoinositide-3-kinase/Akt pathway mediates the transient increase in Nanog expression during differentiation of F9 cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Jung Sun | - |
dc.contributor.author | Kim, Byung Soo | - |
dc.contributor.author | Kim, Jiha | - |
dc.contributor.author | Park, Choon-Sik | - |
dc.contributor.author | Chung, Il Yup | - |
dc.date.accessioned | 2021-06-23T13:03:34Z | - |
dc.date.available | 2021-06-23T13:03:34Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2010-07 | - |
dc.identifier.issn | 0253-6269 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/39671 | - |
dc.description.abstract | Nanog is a key determinant that maintains self-renewal and pluripotency of embryonic stem cells and represses their differentiation to endoderm. In this study, we examined the regulation of Nanog expression by phosphoinositide-3-kinase (PI3K)/Akt pathway during retinoic acid (RA)-induced differentiation of F9 embryonic carcinoma cells. Nanog protein expression was transiently upregulated up to 6 h after RA treatment and then declined. In agreement, a murine Nanog promoter reporter assay revealed that promoter activity increased during early stage of differentiation, but decreased when F9 cells became fully differentiated. RA treatment of F9 cells also led to a transient and parallel increase in both Akt and glycogen synthase kinase 3 beta phosphorylations. Nanog expression was diminished in the early stage by LY294002, a PI3K inhibitor, but was not affected in the late stage despite considerable inhibition of Akt phosphorylation and endoderm marker expression by the inhibitor. These data suggest that RA-induced PI3K/Akt activation in the early stage of differentiation is required for Nanog expression, which becomes independent of PI3K/Akt signaling once the differentiation is established. Thus, Nanog expression appears to be differently regulated by the PI3K/Akt pathway depending on differentiation stage. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | 대한약학회 | - |
dc.title | The phosphoinositide-3-kinase/Akt pathway mediates the transient increase in Nanog expression during differentiation of F9 cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chung, Il Yup | - |
dc.identifier.doi | 10.1007/s12272-010-0719-y | - |
dc.identifier.scopusid | 2-s2.0-77955407169 | - |
dc.identifier.wosid | 000280376400020 | - |
dc.identifier.bibliographicCitation | Archives of Pharmacal Research, v.33, no.7, pp.1117 - 1125 | - |
dc.relation.isPartOf | Archives of Pharmacal Research | - |
dc.citation.title | Archives of Pharmacal Research | - |
dc.citation.volume | 33 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 1117 | - |
dc.citation.endPage | 1125 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001464965 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Medicinal | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | EMBRYONIC STEM-CELLS | - |
dc.subject.keywordPlus | ENDODERM DIFFERENTIATION | - |
dc.subject.keywordPlus | RETINOIC ACID | - |
dc.subject.keywordPlus | PRIMITIVE ENDODERM | - |
dc.subject.keywordPlus | CARCINOMA-CELLS | - |
dc.subject.keywordPlus | ES CELLS | - |
dc.subject.keywordPlus | PLURIPOTENCY | - |
dc.subject.keywordPlus | MAINTENANCE | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | INHIBITOR | - |
dc.subject.keywordAuthor | Embryonic stem cells | - |
dc.subject.keywordAuthor | Endoderm | - |
dc.subject.keywordAuthor | F9 cells | - |
dc.subject.keywordAuthor | Nanog | - |
dc.subject.keywordAuthor | Phosphoinositide-3-kinase | - |
dc.subject.keywordAuthor | Retinoic acid | - |
dc.identifier.url | https://link.springer.com/article/10.1007/s12272-010-0719-y | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
55 Hanyangdeahak-ro, Sangnok-gu, Ansan, Gyeonggi-do, 15588, Korea+82-31-400-4269 sweetbrain@hanyang.ac.kr
COPYRIGHT © 2021 HANYANG UNIVERSITY. ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.