Inhibitory Effects of Deoxypodophyllotoxin from Anthriscus sylvestris on Human CYP2C9 and CYP3A4
DC Field | Value | Language |
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dc.contributor.author | Lee, Sang Kyu | - |
dc.contributor.author | Kim, Yoon | - |
dc.contributor.author | Jin, Changbae | - |
dc.contributor.author | Lee, Seung Ho | - |
dc.contributor.author | Kang, Mi Jeong | - |
dc.contributor.author | Jeong, Tae Cheon | - |
dc.contributor.author | Jeong, Seo Young | - |
dc.contributor.author | Kim, Dong-Hyun | - |
dc.contributor.author | Yoo, Hye Hyun | - |
dc.date.accessioned | 2021-06-23T13:06:24Z | - |
dc.date.available | 2021-06-23T13:06:24Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2010-05 | - |
dc.identifier.issn | 0032-0943 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/39838 | - |
dc.description.abstract | Deoxypodophyllotoxin (DPT) is a bioactive compound of Anthriscus sylvestris (Apiaceae). In the present study, the inhibition of cytochrome P450 (CYP) by DPT was evaluated in human liver microsomes (HLM) and the baculovirus-insect cell-expressed human CYPs using a cocktail probe assay. When a mixture of specific CYP substrates was incubated with DPT in HLM, CYP2C9-catalyzed diclofenac 4-hydroxylation and CYP3A4-catalyzed midazolam 1-hydroxylation were strongly inhibited by DPT, with IC(50) values of 6.3 and 9.2 mu M, respectively. The Lineweaver-Burke plots for the inhibition of CYP2C9 and CYP3A4 in HLM and baculovirus-insect cell-expressed human CYPs were consistent with a competitive type of inhibition. From these results, DPT was characterized to be a competitive inhibitor of CYP2C9 and CYP3A4, with Ki values of 3.5 and 10.8 mu M in HLM and 24.9 and 3.5 mu M in baculovirus-insect cell-expressed human CYPs, respectively. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Georg Thieme Verlag | - |
dc.title | Inhibitory Effects of Deoxypodophyllotoxin from Anthriscus sylvestris on Human CYP2C9 and CYP3A4 | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Yoo, Hye Hyun | - |
dc.identifier.doi | 10.1055/s-0029-1240657 | - |
dc.identifier.scopusid | 2-s2.0-77952046453 | - |
dc.identifier.wosid | 000277659000010 | - |
dc.identifier.bibliographicCitation | Planta Medica, v.76, no.7, pp.701 - 704 | - |
dc.relation.isPartOf | Planta Medica | - |
dc.citation.title | Planta Medica | - |
dc.citation.volume | 76 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 701 | - |
dc.citation.endPage | 704 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalResearchArea | Integrative & Complementary Medicine | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Medicinal | - |
dc.relation.journalWebOfScienceCategory | Integrative & Complementary Medicine | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | CYTOCHROME-P450 3A4 | - |
dc.subject.keywordPlus | METHYLENEDIOXYPHENYL LIGNANS | - |
dc.subject.keywordPlus | MASS-SPECTROMETRY | - |
dc.subject.keywordPlus | COMPLEX-FORMATION | - |
dc.subject.keywordPlus | LIVER-MICROSOMES | - |
dc.subject.keywordPlus | PROBE | - |
dc.subject.keywordPlus | BIOCONVERSION | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | RAT | - |
dc.subject.keywordAuthor | deoxypodophyllotoxin | - |
dc.subject.keywordAuthor | cytochrome P450 | - |
dc.subject.keywordAuthor | competitive inhibition | - |
dc.subject.keywordAuthor | cocktail probe assay | - |
dc.subject.keywordAuthor | Anthriscus sylvestris | - |
dc.subject.keywordAuthor | Apiaceae | - |
dc.identifier.url | https://www.thieme-connect.de/products/ejournals/abstract/10.1055/s-0029-1240657 | - |
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