A relationship between miRNA and gene expression in the mouse Sertoli cell line after exposure to bisphenol A

Abstract

Presently, it is important to address the harmful effects of environmental chemicals on living organisms. The toxic effects of bisphenol A (BPA), one of the endocrine disruption chemicals (EDCs), have been documented in many studies since the 1930s. However, BPA has continued to be used to make polycarbonate plastic, etc. Therefore, it is necessary to conduct toxicogenomic studies to assess the harmful effects of BPA. In this study, microarray technology was used to study the harmful effects format the genomic level. We used two types of microarray chips to study of the relationship between gene and miRNA expression profiles, the Agilent mouse genome 4 x 44 K array for gene expression profiling and the Agilent mouse miRNA v13 for miRNA expression profiling. As a result, we identified 37 miRNAs that were 2-fold up- or down-regulated within 24 hrs than 3 hrs of BPA treat times. The gene expression patterns related to miRNAS were classified into a total of 4 groups. The first and third groups and the second and fourth groups had similar functions to each other as determined by related gene expression. In the first and third groups included overexpressed genes that were related to metabolism. The second and fourth groups included overexpressed genes that were related to reproduction. miRNA expression levels were exchanged for BPA degradation. So, genes of related metabolism were overexpressed. This result was occurred the side effect that was down-expression of reproduction. Thus, miRNAs were regulated by BPA, and gene expression was subsequently regulated by miRNAs.