A Validated LC Method for Simultaneous Determination of Phenolic, Coumarin and Phthalide Compounds in the Ethanolic Extract of Angelica tenuissima
- Authors
- Islam, M. Nurul; Nam, Joo-Won; Lee, Jun; Seo, Eun-Kyoung; Han, Sang Beom; Kim, Dong-Hyun; Jin, Changbae; Yoo, Hye Hyun
- Issue Date
- Oct-2009
- Publisher
- Elsevier BV
- Keywords
- Column liquid chromatography; Phenolic acids; Coumarins and phthalides; Angelica tenuissima
- Citation
- Chromatographia, v.70, no.7-8, pp.1079 - 1085
- Indexed
- SCOPUS
- Journal Title
- Chromatographia
- Volume
- 70
- Number
- 7-8
- Start Page
- 1079
- End Page
- 1085
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/40865
- DOI
- 10.1365/s10337-009-1263-0
- ISSN
- 0009-5893
- Abstract
- In this study, an LC method for the simultaneous determination of six bioactive compounds from Angelica tenuissima, namely chlorogenic acid, ferulic acid, Z-ligustilide, nodakenin, decursin and decursinol angelate was developed and validated. Chromatographic analysis was carried out on a C-18 column with a mobile phase consisting of 0.1% formic acid, methanol and acetonitrile at a flow rate of 0.8 mL min(-1) and the effluent from the column was monitored by UV detector at 325 nm. The excellent linear behavior was observed over the investigated concentration range for reported compounds. The intra- and inter-day precision over the concentration range of compounds were lower than 1.7% (as relative standard deviation), and accuracy was between 97.2 and 106.0%. These results showed that the developed method is accurate, reproducible, and consequently applicable for the quantitation of bioactive components from the ethanolic extract of Angelica tenuissima Nakai.
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