Polo-Like Kinase 1 Depletion Induces DNA Damage in Early S Prior to Caspase Activation
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yim, Hyungshin | - |
dc.contributor.author | Erikson, Raymond L. | - |
dc.date.accessioned | 2021-06-23T15:38:35Z | - |
dc.date.available | 2021-06-23T15:38:35Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2009-05 | - |
dc.identifier.issn | 0270-7306 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/41200 | - |
dc.description.abstract | Polo-like kinase 1 (Plk1) plays several roles in mitosis, and it has been suggested to have a role in tumorigenesis. We have previously reported that Plk1 depletion results in cell death in cancer cells, whereas normal cells survive similar depletion. However, Plk1 depletion together with p53 depletion induces cell death in normal cells as well. This communication presents evidence on the sequence of events that leads to cell death in cancer cells. DNA damage is detected at the first S phase following Plk1 depletion and is more severe in Plk1-depleted p53-null cancer cells. As a consequence of Plk1 depletion using lentivirus-based small interfering RNA techniques, prereplicative complex (pre-RC) formation is disrupted at the G(1)/S transition, and DNA synthesis is reduced during S phase of the first cycle after depletion. The levels of geminin, an inhibitor of DNA pre-RC, and Emi1, an inhibitor of anaphase-promoting complex/cyclosome, are elevated in Plk1-depleted cells. The rate of cell cycling is slower in Plk1-depleted cells than in control cells when synchronized by serum starvation. Plk1 depletion results in disrupted DNA pre-RC formation, reduced DNA synthesis, and DNA damage before cells display severe mitotic catastrophe or apoptosis. Our data suggest that Plk1 is required for cell cycle progression not only in mitosis but also for DNA synthesis, maintenance of DNA integrity, and prevention of cell death. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | AMER SOC MICROBIOLOGY | - |
dc.title | Polo-Like Kinase 1 Depletion Induces DNA Damage in Early S Prior to Caspase Activation | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Yim, Hyungshin | - |
dc.identifier.doi | 10.1128/MCB.01277-08 | - |
dc.identifier.scopusid | 2-s2.0-66349094331 | - |
dc.identifier.wosid | 000265665900011 | - |
dc.identifier.bibliographicCitation | MOLECULAR AND CELLULAR BIOLOGY, v.29, no.10, pp.2609 - 2621 | - |
dc.relation.isPartOf | MOLECULAR AND CELLULAR BIOLOGY | - |
dc.citation.title | MOLECULAR AND CELLULAR BIOLOGY | - |
dc.citation.volume | 29 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 2609 | - |
dc.citation.endPage | 2621 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | ANAPHASE PROMOTING COMPLEX | - |
dc.subject.keywordPlus | PLK1 | - |
dc.subject.keywordPlus | REPLICATION | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | P73 | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | ATM | - |
dc.subject.keywordPlus | DESTRUCTION | - |
dc.subject.keywordPlus | PROGRESSION | - |
dc.subject.keywordPlus | DROSOPHILA | - |
dc.subject.keywordAuthor | ANAPHASE PROMOTING COMPLEX | - |
dc.subject.keywordAuthor | CANCER-CELLS | - |
dc.subject.keywordAuthor | REPLICATION | - |
dc.subject.keywordAuthor | PLK1 | - |
dc.subject.keywordAuthor | APOPTOSIS | - |
dc.subject.keywordAuthor | P73 | - |
dc.subject.keywordAuthor | PHOSPHORYLATION | - |
dc.subject.keywordAuthor | EMI1 | - |
dc.subject.keywordAuthor | ATM | - |
dc.subject.keywordAuthor | DESTRUCTION | - |
dc.identifier.url | https://journals.asm.org/doi/10.1128/MCB.01277-08 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
55 Hanyangdeahak-ro, Sangnok-gu, Ansan, Gyeonggi-do, 15588, Korea+82-31-400-4269 sweetbrain@hanyang.ac.kr
COPYRIGHT © 2021 HANYANG UNIVERSITY. ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.