Co-oxidation-mediated xenobiotic activation and cytotoxicity by 12-lipoxygenase in intact platelets
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lim, Kyung-Min | - |
dc.contributor.author | Kim, Jung-Sun | - |
dc.contributor.author | Bae, Ok-Nam | - |
dc.contributor.author | Noh, Ji-Yoon | - |
dc.contributor.author | Chung, Seung-Min | - |
dc.contributor.author | Chung, Ka-Young | - |
dc.contributor.author | Chung, Jin-Ho | - |
dc.date.accessioned | 2021-06-23T17:39:36Z | - |
dc.date.available | 2021-06-23T17:39:36Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2008-05 | - |
dc.identifier.issn | 0300-483X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/42451 | - |
dc.description.abstract | Peroxidase-mediated co-oxidation process has been suggested as a major alternative pathway for xenobiotic bioactivation other than hepatic cytochrome P450 monooxygenase system. Despite the wealth of reports on the possible involvement of co-oxidation in bioactivation of various compounds, clear manifestation of co-oxidation-mediated xenobiotic bioactivation in intact cell system without extra sources of peroxidase system has been difficult to demonstrate, mainly due to the natural scarcity of peroxidase activities in fresh intact cells. in the present study, arachidonic acid (AA) dependent bioactivation of a-naphthol, a representative phenolic compound, was demonstrated as shown by covalent binding increases in alpha-naphthol concentration dependent manner in freshly prepared intact platelets, where two AA dependent representative peroxidases, 12-lipoxygenase (12-LOX) and prostaglandin H synthase (PHS) are abundantly expressed. Inhibitors of 12-LOX attenuated the covalent binding of alpha-naphthol while inhibitors of PHS were not effective, indicating the predominant role of 12-LOX in AA-initiated co-oxidation in intact platelets. In addition, free radical scavengers and thiol donors prevented effectively the bioactivation of a-naphthol, suggesting the involvement of naphthoxy radical or naphthoxy-derived radical generation. Notably, the co-oxidation process resulted in enhanced cytotoxicities of alpha-naphthol against platelets indeed, as observed by cellular membrane disturbance and mitochondrial membrane potential decrease. With these results, we believe that an important in vitro evidence of peroxidase-mediated xenobiotic activation was provided for understanding the toxicological implication of peroxidase-mediated co-oxidation in the xenobiotic bioactivation. (C) 2008 Elsevier Ireland Ltd. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Elsevier BV | - |
dc.title | Co-oxidation-mediated xenobiotic activation and cytotoxicity by 12-lipoxygenase in intact platelets | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Bae, Ok-Nam | - |
dc.identifier.doi | 10.1016/j.tox.2008.02.015 | - |
dc.identifier.scopusid | 2-s2.0-42749089530 | - |
dc.identifier.wosid | 000256208400012 | - |
dc.identifier.bibliographicCitation | Toxicology, v.247, no.2-3, pp.154 - 160 | - |
dc.relation.isPartOf | Toxicology | - |
dc.citation.title | Toxicology | - |
dc.citation.volume | 247 | - |
dc.citation.number | 2-3 | - |
dc.citation.startPage | 154 | - |
dc.citation.endPage | 160 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalResearchArea | Toxicology | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Toxicology | - |
dc.subject.keywordPlus | PROSTAGLANDIN-H SYNTHASE | - |
dc.subject.keywordPlus | SOYBEAN LIPOXYGENASE | - |
dc.subject.keywordPlus | METABOLIC-ACTIVATION | - |
dc.subject.keywordPlus | PROOXIDANT ACTIVITY | - |
dc.subject.keywordPlus | HUMAN LUNG | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | INHIBITORS | - |
dc.subject.keywordPlus | RADICALS | - |
dc.subject.keywordPlus | ACID | - |
dc.subject.keywordPlus | 1-NAPHTHOL | - |
dc.subject.keywordAuthor | co-oxidation | - |
dc.subject.keywordAuthor | peroxidase-mediated bioactivation | - |
dc.subject.keywordAuthor | cytotoxicity | - |
dc.subject.keywordAuthor | alpha-naphthol | - |
dc.subject.keywordAuthor | reactive metabolite | - |
dc.subject.keywordAuthor | lipoxygenase | - |
dc.subject.keywordAuthor | prostaglandin H synthase | - |
dc.subject.keywordAuthor | platelets | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0300483X08001054?via%3Dihub | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
55 Hanyangdeahak-ro, Sangnok-gu, Ansan, Gyeonggi-do, 15588, Korea+82-31-400-4269 sweetbrain@hanyang.ac.kr
COPYRIGHT © 2021 HANYANG UNIVERSITY. ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.