Functionalized ultra-fine bimetallic PtRu alloy nanoparticle with high peroxidase-mimicking activity for rapid and sensitive colorimetric quantification of C-reactive protein
DC Field | Value | Language |
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dc.contributor.author | Gupta, Pramod K. | - |
dc.contributor.author | Son, Seong Eun | - |
dc.contributor.author | Seong, Gi Hun | - |
dc.date.accessioned | 2021-06-22T04:25:31Z | - |
dc.date.available | 2021-06-22T04:25:31Z | - |
dc.date.issued | 2021-03 | - |
dc.identifier.issn | 0026-3672 | - |
dc.identifier.issn | 1436-5073 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/429 | - |
dc.description.abstract | The in situ synthesis is reported of citric acid-functionalized ultra-fine bimetallic PtRu alloy nanoparticles (CA@PtRu ANPs) through a simple one-pot wet chemical method. The cost-efficient CA@PtRu ANPs with an average diameter of 3.2 nm revealed to have enhanced surface area, peroxidase-like activity, high stability, and adequate availability of functional groups to bind biomolecules. Along with nanoparticle surface area, the surface charge has also significantly affected the peroxidase-like activity and the colloidal suspension stability. As an excellent immobilization matrix and peroxidase mimic, the CA@PtRu ANPs were utilized to develop non-enzymatic colorimetric immunoassay for rapid, selective, and sensitive quantification of C-reactive protein (CRP) biomarkers. In this immunoassay, CA@PtRu ANPs serve as enzyme mimic that significantly amplifies the color signals, and amine-functionalized silica-coated magnetic microbeads (APTES/SiO2@Fe3O4) act as CRP-recognizing capture probes. The absorbance curves of colorimetric immunoassay were measured in wavelengths between 550 and 750 nm, and the maximum absorbance at 652 nm was used to establish a linear relationship between absorbance and CRP concentrations. The developed colorimetric immunoassay showed rapid and sensitive quantification of CRP levels from 0.01 to 180 mu g mL(-1) with a LOD of 0.01 mu g mL(-1). Moreover, the mean recovery of CRP from spiked human serum samples lies between 97 and 109% (n = 3), which indicates that the proposed nanozyme-linked immunoassay has the potential to be used in rapid point-of-care applications. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | SPRINGER WIEN | - |
dc.title | Functionalized ultra-fine bimetallic PtRu alloy nanoparticle with high peroxidase-mimicking activity for rapid and sensitive colorimetric quantification of C-reactive protein | - |
dc.type | Article | - |
dc.publisher.location | 오스트리아 | - |
dc.identifier.doi | 10.1007/s00604-021-04775-4 | - |
dc.identifier.scopusid | 2-s2.0-85102345295 | - |
dc.identifier.wosid | 000627850900003 | - |
dc.identifier.bibliographicCitation | MICROCHIMICA ACTA, v.188, no.4, pp 1 - 10 | - |
dc.citation.title | MICROCHIMICA ACTA | - |
dc.citation.volume | 188 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 10 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | LABEL-FREE DETECTIONCATALYTIC-ACTIVITYCRPNANOMATERIALSIMMUNOASSAYBIOSENSORGOLDNANOSTRUCTURESINFLAMMATIONNANOZYME | - |
dc.subject.keywordAuthor | Bimetallic alloy | - |
dc.subject.keywordAuthor | Nanozyme | - |
dc.subject.keywordAuthor | Immunoassay | - |
dc.subject.keywordAuthor | C-reactive protein | - |
dc.subject.keywordAuthor | Colorimetric detection | - |
dc.identifier.url | https://link.springer.com/article/10.1007/s00604-021-04775-4 | - |
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