Molecular cloning and characterization of omega class glutathione S-transferase (GST-O) from the polychaete Neanthes succinea: Biochemical comparison with theta class glutathione S-transferase (GST-T)
DC Field | Value | Language |
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dc.contributor.author | Rhee, Jae-Sung | - |
dc.contributor.author | Lee, Young-Mi | - |
dc.contributor.author | Hwang, Dae-Sik | - |
dc.contributor.author | Lee, Kyun-Woo | - |
dc.contributor.author | Kim, Il-Chan | - |
dc.contributor.author | Shin, Kyung-Hoon | - |
dc.contributor.author | Raisuddin, Sheikh | - |
dc.contributor.author | Lee, Jae-Seong | - |
dc.date.accessioned | 2021-06-23T19:04:07Z | - |
dc.date.available | 2021-06-23T19:04:07Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2007-11 | - |
dc.identifier.issn | 1532-0456 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/43322 | - |
dc.description.abstract | We cloned and sequenced a full-length cDNA of an omega class glutathione S-transferase (GST-O) from the polychaete Neanthes succinea (ns-GST-O). The full-length cDNA of ns-GST-O was 1562 bp in length, containing an open reading frame (OR) of 732 bp that encoded a 244 amino acid protein. The deduced amino acid sequence of ns-GST-O showed a low similarity with the theta class N. suucinea GST (ns-GST-T). As GSTs play a significant role in antioxidant defense, we checked the expression pattern of ns-GST-0 in N. succinea after exposure to copper (CUCl2 12 to 72 mu g/L), which is an oxidative stress-inducing agent. After exposure to CUCl2, ns-GST-O gene was dramatically up-regulated and when compared with ns-GST-T the expression pattern was more pronounced at all the concentrations of copper. Even the basal transcription levels of ns-GST-O were higher than those of ns-GST-T To further characterize the catalytic properties of ns-GST-O, we constructed a recombinant ns-GST-O plasmid with a 6x His-Tag at the N-terminal of the full-length ns-GST-O cDNA. Recombinant ns-GST-O protein was highly expressed in transformed Escherichia coli. The effect of pH, temperature and chemical inhibitors on the enzyme activity of ns-GST-O was also studied and compared with the reported effect of these factors on recombinant ns-GST-T protein. These results suggest that, like other types of GSTs, ns-GST-O protein plays a conserved antioxidant role in the polychacte N. succinea. (C) 2007 Elsevier Inc. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Elsevier BV | - |
dc.title | Molecular cloning and characterization of omega class glutathione S-transferase (GST-O) from the polychaete Neanthes succinea: Biochemical comparison with theta class glutathione S-transferase (GST-T) | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Shin, Kyung-Hoon | - |
dc.identifier.doi | 10.1016/j.cbpc.2007.05.003 | - |
dc.identifier.scopusid | 2-s2.0-34848858489 | - |
dc.identifier.wosid | 000250666100003 | - |
dc.identifier.bibliographicCitation | Comparative Biochemistry and Physiology, Part C, v.146, no.4, pp.471 - 477 | - |
dc.relation.isPartOf | Comparative Biochemistry and Physiology, Part C | - |
dc.citation.title | Comparative Biochemistry and Physiology, Part C | - |
dc.citation.volume | 146 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 471 | - |
dc.citation.endPage | 477 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Endocrinology & Metabolism | - |
dc.relation.journalResearchArea | Toxicology | - |
dc.relation.journalResearchArea | Zoology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Endocrinology & Metabolism | - |
dc.relation.journalWebOfScienceCategory | Toxicology | - |
dc.relation.journalWebOfScienceCategory | Zoology | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | RESISTANCE | - |
dc.subject.keywordPlus | TOXICITY | - |
dc.subject.keywordPlus | DETOXIFICATION | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordAuthor | polychaete | - |
dc.subject.keywordAuthor | Neanthes succinea | - |
dc.subject.keywordAuthor | glutathione S-transferases | - |
dc.subject.keywordAuthor | antioxidant defense | - |
dc.subject.keywordAuthor | biomarker | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S153204560700138X?via%3Dihub | - |
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