PDMS-glass serpentine microchannel chip for time domain PCR with bubble suppression in sample injection
DC Field | Value | Language |
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dc.contributor.author | Cho, Chul-Ho | - |
dc.contributor.author | Cho, Woong | - |
dc.contributor.author | Ahn, Yoomin | - |
dc.contributor.author | Hwang, Seung-Yong | - |
dc.date.accessioned | 2021-06-23T19:06:31Z | - |
dc.date.available | 2021-06-23T19:06:31Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2007-09 | - |
dc.identifier.issn | 0960-1317 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/43468 | - |
dc.description.abstract | This paper reports on the development of a low-cost microreactor ( 10 mu l) biochip for DNA polymerase chain reaction ( PCR). The microbiochip ( 20 mm x 28 mm) is a hybrid type that is composed of a polydimethylsiloxane ( PDMS) layer with a serpentine microchannel ( 360 mu m x 100 mu m) chamber and glass substrate integrated with a microheater and thermal microsensor. Because of the hydrophobic chip surface, bubbles are usually created during sample loading in the PMDS-based microchip. These bubbles disrupt the stable biochemical reaction. An improved microreactor chamber was designed using microfluidic simulation. The reactor has a rounded-corner serpentine channel architecture, which enables stable injection into the hydrophobic surface using only a micropipette. The reactor temperature needed for the PCR reaction is controlled within +/- 0.5 degrees C by the LabVIEW software proportional-integrative-derivative (PID) controller. PCR analyses of the sex-determining Y chromosome (SRY) gene and mouse GAPDH gene were successfully performed in less than 54 min by the fabricated microreactor chip. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Institute of Physics Publishing | - |
dc.title | PDMS-glass serpentine microchannel chip for time domain PCR with bubble suppression in sample injection | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Ahn, Yoomin | - |
dc.contributor.affiliatedAuthor | Hwang, Seung-Yong | - |
dc.identifier.doi | 10.1088/0960-1317/17/9/009 | - |
dc.identifier.scopusid | 2-s2.0-50949124660 | - |
dc.identifier.wosid | 000249222400020 | - |
dc.identifier.bibliographicCitation | Journal of Micromechanics and Microengineering, v.17, no.9, pp.1810 - 1817 | - |
dc.relation.isPartOf | Journal of Micromechanics and Microengineering | - |
dc.citation.title | Journal of Micromechanics and Microengineering | - |
dc.citation.volume | 17 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 1810 | - |
dc.citation.endPage | 1817 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Instruments & Instrumentation | - |
dc.relation.journalResearchArea | Physics | - |
dc.relation.journalWebOfScienceCategory | Engineering, Electrical & Electronic | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Instruments & Instrumentation | - |
dc.relation.journalWebOfScienceCategory | Physics, Applied | - |
dc.subject.keywordPlus | POLYMERASE-CHAIN-REACTION | - |
dc.subject.keywordPlus | CONTINUOUS-FLOW PCR | - |
dc.subject.keywordPlus | DNA AMPLIFICATION | - |
dc.subject.keywordPlus | HYBRID MICROCHIP | - |
dc.subject.keywordPlus | ELECTROPHORESIS | - |
dc.identifier.url | https://iopscience.iop.org/article/10.1088/0960-1317/17/9/009 | - |
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