Molecular cloning, expression, biochemical characteristics, and biomarker potential of theta class glutathione S-transferase (GST-T) from the polychaete Neanthes succinea
DC Field | Value | Language |
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dc.contributor.author | Rhee, Jae-Sung | - |
dc.contributor.author | Lee, Young-Mi | - |
dc.contributor.author | Hwang, Dae-Sik | - |
dc.contributor.author | Won, Eun-Ji | - |
dc.contributor.author | Raisuddin, Sheikh | - |
dc.contributor.author | Shin, Kyung-Hoon | - |
dc.contributor.author | Lee, Jae-Seong | - |
dc.date.accessioned | 2021-06-23T19:39:05Z | - |
dc.date.available | 2021-06-23T19:39:05Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2007-06 | - |
dc.identifier.issn | 0166-445X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/43625 | - |
dc.description.abstract | We cloned and sequenced the full-length cDNA of a theta class glutathione S-transferase (GST-T) from the polychaete Neanthes succinea. The open reading frame of N. succinea GST-T cDNA was 678 bp and encoded 226 amino acid residues. We generated recombinant N. succinea GST-T by expression in transformed Escherichia coli and studied the kinetic properties as well as the effects of inhibitors, pH, and temperature on N. succinea GST-T. GST-T expression was studied using real-time RT-PCR in response to exposure to the model oxidative stress-inducing agent, CuCl2. Copper induced a concentration-dependant increase in the expression of GST-T. Moreover, polychaetes collected from a heavily contaminated lake near an industrial complex showed significantly higher levels of GST-T expression. Interestingly, the site-collected polychaetes with the highest GST-T mRNA expression levels also showed the highest metallothioneins levels. These results suggest that GST-T in polychaetes may have an antioxidant role and that N. succinea GST-T expression may be a useful biomarker for exposure to environmental contaminants such as copper. Our findings provide a better understanding of the biochemical characteristics of N. succinea GST-T, and elucidate the potential role of GST-T in heavy metal-induced oxidative stress and as a biomarker for environmental contamination. (c) 2007 Elsevier B.V. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Elsevier BV | - |
dc.title | Molecular cloning, expression, biochemical characteristics, and biomarker potential of theta class glutathione S-transferase (GST-T) from the polychaete Neanthes succinea | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Shin, Kyung-Hoon | - |
dc.identifier.doi | 10.1016/j.aquatox.2007.03.015 | - |
dc.identifier.scopusid | 2-s2.0-34248586715 | - |
dc.identifier.wosid | 000247556100003 | - |
dc.identifier.bibliographicCitation | Aquatic Toxicology, v.83, no.2, pp.104 - 115 | - |
dc.relation.isPartOf | Aquatic Toxicology | - |
dc.citation.title | Aquatic Toxicology | - |
dc.citation.volume | 83 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 104 | - |
dc.citation.endPage | 115 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Marine & Freshwater Biology | - |
dc.relation.journalResearchArea | Toxicology | - |
dc.relation.journalWebOfScienceCategory | Marine & Freshwater Biology | - |
dc.relation.journalWebOfScienceCategory | Toxicology | - |
dc.subject.keywordPlus | FISH RIVULUS-MARMORATUS | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | NEARBY AREAS | - |
dc.subject.keywordPlus | KYEONGGI BAY | - |
dc.subject.keywordPlus | LAKE SHIHWA | - |
dc.subject.keywordPlus | RESPONSES | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | METALLOTHIONEINS | - |
dc.subject.keywordPlus | BIOACCUMULATION | - |
dc.subject.keywordPlus | SUSCEPTIBILITY | - |
dc.subject.keywordAuthor | polychaete | - |
dc.subject.keywordAuthor | neanthes succinea | - |
dc.subject.keywordAuthor | glutathione S-transferases | - |
dc.subject.keywordAuthor | heavy metals | - |
dc.subject.keywordAuthor | metallothioneins | - |
dc.subject.keywordAuthor | oxidative stress | - |
dc.subject.keywordAuthor | biomarker | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0166445X07001154?via%3Dihub | - |
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