Surface-enhanced Raman spectroscopic-encoded beads for multiplex immunoassay
- Authors
- Jun, Bong-Hyun; Kim, Jong-Ho; Park, Hyunmi; Kim, Jun-Sung; Yu, Kyeong-Nam; Lee, Sang-Myung; Choi, Heejeong; Kwak, Seon-Yeong; Kim, Yong-Kweon; Jeong, Dae Hong; Cho, Myung-Haing; Lee, Yoon-Sik
- Issue Date
- Mar-2007
- Publisher
- American Chemical Society
- Keywords
- PERFORMANCE LIQUID-CHROMATOGRAPHY; MONODISPERSE POLYMER BEADS; BUNDLE IMAGE COMPRESSION; SOLID-PHASE REACTIONS; COMBINATORIAL CHEMISTRY; SENSITIVE DETECTION; ORGANIC-SYNTHESIS; PACKING MATERIAL; SCATTERING; NANOPARTICLES
- Citation
- Journal of Combinatorial Chemistry, v.9, no.2, pp.237 - 244
- Indexed
- SCIE
SCOPUS
- Journal Title
- Journal of Combinatorial Chemistry
- Volume
- 9
- Number
- 2
- Start Page
- 237
- End Page
- 244
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/43845
- DOI
- 10.1021/cc0600831
- ISSN
- 1520-4766
- Abstract
- A new type of encoded bead, which uses surface-enhanced Raman scattering (SERS), is described for multiplex immunoassays. Silver nanoparticles were embedded in sulfonated polystyrene (PS) beads via a polyol method, and they were used as SERS-active substrates. Raman-label organic compounds such as 4-methylbenzenethiol (4-MT), 2-naphthalenethiol (2-NT), and benzenethiol (BT) were then adsorbed onto the silver nanoparticles in the sulfonated PS bead. Although only three kinds of encoding have been demonstrated here, various combinations of these Raman-label organic compounds have the potential to give a large number of tags. The Raman-label-incorporated particles were then coated with a silica shell using tetraethoxyorthosilicate (TEOS) for chemical stability and biocompatibility. The resulting beads showed unique and intense Raman signals for the labeled organic compounds. We demonstrated that SERS-encoded beads could be used for multiplex detection with a model using streptavidin and p53. In our system, the binding event of target molecules and the type of ligand can be simultaneously recognized by Raman spectroscopy using a single laser-line excitation (514.5 nm).
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Collections - COLLEGE OF ENGINEERING SCIENCES > DEPARTMENT OF MATERIALS SCIENCE AND CHEMICAL ENGINEERING > 1. Journal Articles
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