Interaction of ε-L-β-lysine as a tail analogy of tallysomycin-A to a double helical DNA oligonucletide d(CGCTTCGAAGCG)2

Abstract

During the screening of material which has the antimicrobial activity against aminoglycoside-resistant bacteria, A new material -(L-β-lysine) polypeptide from a culture medium of Streptomyces sp.(DWGS2) was isolated, and the structure and the physicochemical properties of the new material were elucidated. The new material was separated by column chromatography of the culture medium using Dowex1×2, Silica gel, and Sephadex LH20 etc. The structure and molecular weight were determined with the data of NMR, MALDI mass, and ESI mass experiments. And the monomer obtained by hydrolysis of the new material with 6N-HCl was identified as a L-β-lysine(T2), which is a tail of bleomycin. As tail-region analogy, T2(β-lysine derivatives from streptomyces) interactions with a self-complementary oligonucleotides, d(CGCTTCGAAGCG)2, was investigated by NMR.