FRET에 기반한 Open Sandwich Fluoroimmunoassay
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Quande Wei | - |
dc.contributor.author | 이문권 | - |
dc.contributor.author | 성기훈 | - |
dc.contributor.author | 주재범 | - |
dc.contributor.author | 이은규 | - |
dc.date.accessioned | 2021-06-23T20:06:18Z | - |
dc.date.available | 2021-06-23T20:06:18Z | - |
dc.date.issued | 2007-12 | - |
dc.identifier.issn | 1225-7117 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/43979 | - |
dc.description.abstract | QDs을 기반으로 하는 OsFIA는 매우 빠르고 간단히 수행될 수 있다. 또한 이 분석법은 고체상의 담체나 결합/잔류 시약의 분리 등과 같은 여러 과정을 필요로 하지 않으며, 적은 양의 시약으로도 분석이 가능하다. 본 분석법은 높은 감도로 항원을 측정할 수 있으며, 일상적인 분석에도 쉽게 도입될 수 있을 것이다. 선형 범위 내에서 측정 가능한 receptor의 최소농도는 0.05 nM (2.65 ng/mL) 정도이다. 또한, 일반적으로 상용화된 항체를 가지고 수행이 가능하다. 이 OsFIA 분석법은 기존의 실험적 sandwich immunoassay의 효과적인 대안으로 제시된다. | - |
dc.description.abstract | We have developed a sensitive, one-step, homogeneous open sandwich fluoroimmunoassay (OsFIA) based on fluorescence resonance energy transfer (FRET) and luminescent semiconductor quantum dots (QDs). In this FRET assay, estrogen receptor-β (ER-β) antigen was incubated with QD-labeled anti-ER-β monoclonal antibody and AF (Alexa Fluoro)-labeled anti-ER polyclonal antibody for 30 minutes, followed by FRET measurement. The dye separation distance was estimated to be between 80~90 Å. The present method is rapid, simple and highly sensitive, and did not require the bound/free reagent separation steps and solid-phase carriers. A concentration as low as 0.05 nM (2.65 ng/ml) receptor was detected with linearity (R2 > 0.990). In addition, the assay was performed with commercial antibodies. This assay provides a convenient alternative to conventional, laborious sandwich immunoassays. | - |
dc.format.extent | 7 | - |
dc.language | 한국어 | - |
dc.language.iso | KOR | - |
dc.publisher | 한국생물공학회 | - |
dc.title | FRET에 기반한 Open Sandwich Fluoroimmunoassay | - |
dc.title.alternative | Development of an Open Sandwich FluoroimmunoassayBased on FRET | - |
dc.type | Article | - |
dc.publisher.location | 대한민국 | - |
dc.identifier.bibliographicCitation | KSBB Journal, v.22, no.6, pp 426 - 432 | - |
dc.citation.title | KSBB Journal | - |
dc.citation.volume | 22 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 426 | - |
dc.citation.endPage | 432 | - |
dc.identifier.kciid | ART001214844 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | kci | - |
dc.subject.keywordAuthor | FRET | - |
dc.subject.keywordAuthor | sandwich assay | - |
dc.subject.keywordAuthor | quantum dots | - |
dc.subject.keywordAuthor | fluorescence labels | - |
dc.subject.keywordAuthor | sandwich immunoassay | - |
dc.subject.keywordAuthor | FRET | - |
dc.subject.keywordAuthor | sandwich assay | - |
dc.subject.keywordAuthor | quantum dots | - |
dc.subject.keywordAuthor | fluorescence labels | - |
dc.subject.keywordAuthor | sandwich immunoassay | - |
dc.identifier.url | https://www.earticle.net/Article/A102290 | - |
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