Expression, purification, and antibody binding activity of human papillomavirus 16 L1 protein fused to maltose binding protein
DC Field | Value | Language |
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dc.contributor.author | Cho, Hee-Jeong | - |
dc.contributor.author | Hahm, Moon Sun | - |
dc.contributor.author | Kim, Myung Kuk | - |
dc.contributor.author | Han, In-Kwon | - |
dc.contributor.author | Jung, Woon-Won | - |
dc.contributor.author | Choi, Han-Gon | - |
dc.contributor.author | Kim, Jung Ae | - |
dc.contributor.author | Oh, Yu-Kyoung | - |
dc.date.accessioned | 2021-06-23T20:44:21Z | - |
dc.date.available | 2021-06-23T20:44:21Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2007-03 | - |
dc.identifier.issn | 0929-8665 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/44377 | - |
dc.description.abstract | Genetic human papillornavirus type 16 L1 (HPV16 L1) has been widely studied for cervical cancer vaccine development. For the enzyme-linked immunosorbent assay (ELISA) screening of these vaccines, HPV 16 L1 protein, which is required as a coating protein, has previously been expressed from costly and laborious recombinant baculovirus-infected insect cells. For a novel-HPV16 L1 expression system characterized by a high yield of soluble-form with simple purification steps, we have cloned and expressed two different types of HPV16 L1, both fused to maltose binding protein (MBP) or glutathione-S-transferase (GST) in Escherichia coli. The yield of soluble HPV16 L1 was influenced by the cultivation temperature. The yield of soluble form in the total MBP-fused HPV 16 L1 protein (MBP-HPV16 L1) was 35% at 37 degrees C, but increased to 85% at 22 degrees C. Among the fusion partners, MBP provided higher yields of total and soluble HPV16 L1 than did GST. MBP-HPV16 L1 showed a 4.9-fold higher yield of the soluble form over insoluble inclusion bodies under optimized culture conditions. The soluble form of MBP-HPV16 L1 was purified via MBP affinity chromatography in a recovery yield of 9.7%. After fusion with MBP, HPV16 L1 showed binding activity to HPV16 L1-specific monoclonal antibody comparable to HPV16 L1 from the insect cells in ELISA tests. These results demonstrate that the use of MBP as a fusion partner may generate a high yield of soluble HPV 16 L1 under optimized temperature conditions, and that MBP-fused HPV16 L1 might be applied further in evaluations of the immune responses of HPV16 L1-based cervical cancer vaccines. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | BENTHAM SCIENCE PUBL LTD | - |
dc.title | Expression, purification, and antibody binding activity of human papillomavirus 16 L1 protein fused to maltose binding protein | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Choi, Han-Gon | - |
dc.identifier.doi | 10.2174/092986607780782722 | - |
dc.identifier.scopusid | 2-s2.0-34249740277 | - |
dc.identifier.wosid | 000247415500002 | - |
dc.identifier.bibliographicCitation | PROTEIN AND PEPTIDE LETTERS, v.14, no.5, pp.417 - 424 | - |
dc.relation.isPartOf | PROTEIN AND PEPTIDE LETTERS | - |
dc.citation.title | PROTEIN AND PEPTIDE LETTERS | - |
dc.citation.volume | 14 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 417 | - |
dc.citation.endPage | 424 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | ENHANCED MUCOSAL | - |
dc.subject.keywordPlus | CAPSID PROTEIN | - |
dc.subject.keywordPlus | L1 | - |
dc.subject.keywordPlus | IMMUNOGENICITY | - |
dc.subject.keywordPlus | VACCINES | - |
dc.subject.keywordPlus | SOLUBILITY | - |
dc.subject.keywordPlus | PARTICLES | - |
dc.subject.keywordPlus | PEPTIDE | - |
dc.subject.keywordPlus | ARCHAEA | - |
dc.subject.keywordAuthor | HPV16 L1 protein | - |
dc.subject.keywordAuthor | fusion protein | - |
dc.subject.keywordAuthor | maltose binding protein | - |
dc.subject.keywordAuthor | solubilization | - |
dc.subject.keywordAuthor | ELISA | - |
dc.identifier.url | https://www.eurekaselect.com/78272/article | - |
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