Poly(dimethyl siloxane)-based protein chip for simultaneous detection of multiple samples: Use of glycidyl methacrylate photopolymer for site-specific protein immobilization
DC Field | Value | Language |
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dc.contributor.author | Park, Kyoung Hwan | - |
dc.contributor.author | Park, Hyun Gyu | - |
dc.contributor.author | Kim, Joon-Ho | - |
dc.contributor.author | Seong, Ki Hun | - |
dc.date.accessioned | 2021-06-23T21:02:31Z | - |
dc.date.available | 2021-06-23T21:02:31Z | - |
dc.date.issued | 2006-12 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.issn | 1873-4235 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/44421 | - |
dc.description.abstract | This paper describes fabrication of a poly(dimethyl siloxane) (PDMS)-based chip to analyze multiple protein interactions utilizing glycidyl methacrylate (GMA) photopolymer for a site-specific immobilization of capture proteins in a closed system. First, using one direction channels of a PDMS mold having cross-channels, GMA micropads were prepared by photopolymerizing GMA solution by 365 nm light irradiation at predetermined positions. After the first mold was replaced with a second mold having higher height or directly without mold changing, capture proteins were allowed to be covalently immobilized onto the surface of the epoxide-activated GMA pads. Following immobilization, poly(ethylene glycol) diacrylate (PEG-DA) precursor was photopolymerized at specific regions to generate plugs for prevention of mixing between different sample injection channels, diminishing the need of a mold changing for sample injections. Final chip was assembled by connecting separated sample injection channels using a connector mold. The viability of this strategy was successfully demonstrated by simultaneous detection of two different antigen-antibody interactions. (c) 2006 Elsevier B.V. All rights reserved. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.title | Poly(dimethyl siloxane)-based protein chip for simultaneous detection of multiple samples: Use of glycidyl methacrylate photopolymer for site-specific protein immobilization | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1016/j.bios.2006.01.029 | - |
dc.identifier.scopusid | 2-s2.0-33750969698 | - |
dc.identifier.wosid | 000242230800006 | - |
dc.identifier.bibliographicCitation | BIOSENSORS & BIOELECTRONICS, v.22, no.5, pp 613 - 620 | - |
dc.citation.title | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.volume | 22 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 613 | - |
dc.citation.endPage | 620 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.subject.keywordPlus | MICROFLUIDIC SYSTEMS | - |
dc.subject.keywordPlus | FABRICATION | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | HYDROGELS | - |
dc.subject.keywordPlus | POLYMER | - |
dc.subject.keywordPlus | MICROSTRUCTURES | - |
dc.subject.keywordPlus | MICROARRAY | - |
dc.subject.keywordPlus | MICROCHIP | - |
dc.subject.keywordPlus | ARRAYS | - |
dc.subject.keywordAuthor | glycidyl methacrylate | - |
dc.subject.keywordAuthor | poly(dimethyl siloxane) (PDMS) | - |
dc.subject.keywordAuthor | microfluidics | - |
dc.subject.keywordAuthor | poly(ethylene glycol) diacrylate | - |
dc.subject.keywordAuthor | biomolecular immobilization | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0956566306000509?via%3Dihub | - |
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