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DNA의 제한효소 반응 및 전기영동 검출용 PDMS/유리 마이크로바이오칩PDMS/Glass Based DNA Microbiochip for Restriction Enzyme Reaction and Electrophoresis Detection
- Other Titles
- PDMS/Glass Based DNA Microbiochip for Restriction Enzyme Reaction and Electrophoresis Detection
- Issue Date
- Jan-2006
- Publisher
- 대한기계학회
- Keywords
- DNA(디옥시리보핵산); Restriction Enzyme(제한효소); Electrophoresis(전기영동); Microbiochip(초소형 생체칩)
- Citation
- 대한기계학회논문집 A, v.30, no.1, pp 26 - 31
- Pages
- 6
- Indexed
- KCI
- Journal Title
- 대한기계학회논문집 A
- Volume
- 30
- Number
- 1
- Start Page
- 26
- End Page
- 31
- ISSN
- 1226-4873
- Abstract
- This paper reports low-cost PDMS/glass based DNA microbiochip for the restriction enzyme reaction and its products detection using the capillary electrophoresis. The microbiochip (25mm × 75mm) has the heater integrated reactor (5㎕) for DNA restriction enzyme reaction at 37℃ and the microchannel (80㎛x100㎛x58mm) for the capillary electrophoresis detection. It is experimentally confirmed that the digestion of the plasmid (pGEM??-4Z) by the enzyme (Hind III and Sca I) is performed for less than 10 min and its electrophoresis detection is able to sequentially on the fabricated microbiochip.
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Related Researcher
- Hwang, Seung Yong
- ERICA 첨단융합대학 (ERICA 분자의약전공)