Micropatterning of biomolecules on glass surfaces modified with various functional groups using photoactivatable biotin
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Hyunju | - |
dc.contributor.author | Kim, Nam-hyun | - |
dc.contributor.author | Chung, Bong-hyun | - |
dc.contributor.author | Seong, Gi-hoon | - |
dc.date.accessioned | 2021-06-23T22:41:32Z | - |
dc.date.available | 2021-06-23T22:41:32Z | - |
dc.date.issued | 2005-12 | - |
dc.identifier.issn | 0003-2697 | - |
dc.identifier.issn | 1096-0309 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/45461 | - |
dc.description.abstract | Biomolecule patterning by photolithographic methods has considerable advantages because a large number of different biomolecules can be assembled on a spatial area by a combinatorial method and complex biomolecule patterning can be created in situ in closed environments such as microfluidic channels. Here, a photobiotin was used as the photoactivatable reagent to create patterned arrays of biomolecules. The variability of photobiotin deposition on glass substrates modified with a variety of materials having carboxyl, lysine, aldehyde, amine groups, and BSA (bovine serum albumin) was characterized by subsequent derivatization with Cy3-labeled streptavidin. The fluorescence images of the photobiotin patterned glass surfaces showed that the BSA/aldehyde-coated glass could be considered as the most appropriate substrate to immobilize photobiotin, in view of the homogeneous immobilization of biomolecules with high density in defined regions and the reduction of nonspecific binding to the surface. In streptavidin equilibrium adsorption assays, the maximum amount of streptavidin-Cy3 bound to the BSA/aldehyde-coated glass surface continued to rise with increasing streptavidin-Cy3 concentration until 12.0 mu g/mL was reached and the surface then became saturated. Also, a line array of biotin-labeled single-strand probe DNAs was created on the BSA/aldehyde-coated glass by photolysis of photobiotin through a slit-type mask and biotin/streptavidin/biotin chemistry, extended to a quantitative measurement of the concentrations of target DNA. The results of target DNA analysis showed linearity over a wide range from 0.5 ng/mL to 5 mu g/mL and were reproducible. (c) 2005 Elsevier Inc. All rights reserved. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | Micropatterning of biomolecules on glass surfaces modified with various functional groups using photoactivatable biotin | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1016/j.ab.2005.08.015 | - |
dc.identifier.scopusid | 2-s2.0-27744479984 | - |
dc.identifier.wosid | 000233698400008 | - |
dc.identifier.bibliographicCitation | ANALYTICAL BIOCHEMISTRY, v.347, no.1, pp 60 - 66 | - |
dc.citation.title | ANALYTICAL BIOCHEMISTRY | - |
dc.citation.volume | 347 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 60 | - |
dc.citation.endPage | 66 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | DIP-PEN NANOLITHOGRAPHY | - |
dc.subject.keywordPlus | MICROFLUIDIC CHANNELS | - |
dc.subject.keywordPlus | IMMOBILIZATION | - |
dc.subject.keywordPlus | CHEMISTRY | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordAuthor | photobiotin | - |
dc.subject.keywordAuthor | photoactivation | - |
dc.subject.keywordAuthor | micropatterning | - |
dc.subject.keywordAuthor | immobilization | - |
dc.subject.keywordAuthor | DNA hybridization | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0003269705005993?via%3Dihub | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
55 Hanyangdeahak-ro, Sangnok-gu, Ansan, Gyeonggi-do, 15588, Korea+82-31-400-4269 sweetbrain@hanyang.ac.kr
COPYRIGHT © 2021 HANYANG UNIVERSITY. ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.