Induction of apoptosis in human leukemia cells by MCS-C2 via caspase-dependent Bid cleavage and cytochrome c release
DC Field | Value | Language |
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dc.contributor.author | Kim, Min Kyoung | - |
dc.contributor.author | Cho, Youl-Hee | - |
dc.contributor.author | Kim, Jung Mogg | - |
dc.contributor.author | Moon Woo Chun | - |
dc.contributor.author | Lee, Seung Ki | - |
dc.contributor.author | Lim, Yoongho | - |
dc.contributor.author | Lee, Chul-Hoon | - |
dc.date.accessioned | 2021-06-23T23:06:39Z | - |
dc.date.available | 2021-06-23T23:06:39Z | - |
dc.date.issued | 2005-06 | - |
dc.identifier.issn | 0304-3835 | - |
dc.identifier.issn | 1872-7980 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/45872 | - |
dc.description.abstract | The purpose of the present study was to investigate the anti-proliferative and apoptotic effects of MCS-C2, a novel synthetic analogue of the pyrrolo[2,3-d]pyrimidine nucleoside toyocamycin and sangivamycin, in human promyelocytic leukemia (HL-60) cells. When treated with 5 mu M MCS-C2, inhibited proliferation associated with apoptotic induction was found in the HL-60 cells in a concentration-dependent and time-dependent manner, plus nuclear DAPI staining revealed the typical nuclear features of apoptosis. However, MCS-C2 showed almost no antiproliferative effect and no apoptotic induction in normal lymphocyte cells used as a control when compared with those in HL-60 cancer cells. Moreover, a flow cytometric analysis of the HL-60 cells using FITC-dUTP and propidium iodide (PI) showed that the apoptotic cell population increased gradually from < 1% at 0 h to 34% at 12 h after exposure to 5 mu M MCS-C2. This apoptotic induction was associated with the cleavage of Bid and a release of cytochrome c from mitochondria into the cytosol, followed by the activation of caspase-3 and inactivation of poly(ADP-ribose) polymerase (PARP). However, there was no significant change in any other mitochondrial membrane proteins, such as Bcl-2 and Bax. Consequently, the current findings suggest that the mitochondrial pathway was primarily involved in the MCS-C2-induced apoptosis in the human promyelocytic leukemia HL-60 cells. (c) 2004 Elsevier Ireland Ltd. All rights reserved. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Elsevier BV | - |
dc.title | Induction of apoptosis in human leukemia cells by MCS-C2 via caspase-dependent Bid cleavage and cytochrome c release | - |
dc.type | Article | - |
dc.publisher.location | 아일랜드 | - |
dc.identifier.doi | 10.1016/j.canlet.2004.10.045 | - |
dc.identifier.scopusid | 2-s2.0-19344363882 | - |
dc.identifier.wosid | 000229731400007 | - |
dc.identifier.bibliographicCitation | Cancer Letters, v.223, no.2, pp 239 - 247 | - |
dc.citation.title | Cancer Letters | - |
dc.citation.volume | 223 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 239 | - |
dc.citation.endPage | 247 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.subject.keywordPlus | RIBONUCLEIC-ACID SYNTHESIS | - |
dc.subject.keywordPlus | ANTICANCER DRUGS | - |
dc.subject.keywordPlus | POLY(ADP-RIBOSE) POLYMERASE | - |
dc.subject.keywordPlus | ANTIVIRAL ACTIVITY | - |
dc.subject.keywordPlus | SIGNALING COMPLEX | - |
dc.subject.keywordPlus | TOYOCAMYCIN | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | SANGIVAMYCIN | - |
dc.subject.keywordPlus | INHIBITOR | - |
dc.subject.keywordPlus | PROTEASE | - |
dc.subject.keywordAuthor | MCS-C2 | - |
dc.subject.keywordAuthor | leukemia cell | - |
dc.subject.keywordAuthor | cytochrome c | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | bid | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0304383504008547?via%3Dihub | - |
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