Microaffinity purification of proteins based on photolytic elution: Toward an efficient microbead affinity chromatography on a chip
DC Field | Value | Language |
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dc.contributor.author | Chung, Woo jae | - |
dc.contributor.author | Kim, Min su | - |
dc.contributor.author | Cho, Suhyung | - |
dc.contributor.author | Park, Sung soo | - |
dc.contributor.author | Kim, Jong Ho | - |
dc.contributor.author | Kim, Yong kweon | - |
dc.contributor.author | Kim, Byung gee | - |
dc.contributor.author | Lee, Yoon sik | - |
dc.date.accessioned | 2021-06-23T23:40:46Z | - |
dc.date.available | 2021-06-23T23:40:46Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2005-02 | - |
dc.identifier.issn | 0173-0835 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/46113 | - |
dc.description.abstract | A bead affinity chromatography system, which was based on the photolytic elution method, was integrated into a glass-silicon microchip to purify specific target proteins. CutiCore(R) beads, which were coupled with a photo-cleavable ligand, such as biotin and an RNA aptamer, were introduced into a filter chamber in the microchip. The protein mixture containing target protein labeled with fluorescein isothiocyanate (FITC) was then passed through the packed affinity beads in the microchamber by pressure-driven flow. During the process, the adsorbed protein on the bead was monitored by fluorescence. The concentrated target protein on the affinity bead was released by simple irradiation with UV light at a wavelength of 360 nm, and subsequently eluted with the phosphate buffer flow. The eluted target protein was quantitatively detected via the fluorescence intensity measurements at the downstream of the capillary connected to the outlet of the microchip. The microaffinity purification allowed for a successful method for the identification of specific target proteins from a protein mixture. In addition, the feasibility of this system for use as a diagnosis chip was demonstrated. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | John Wiley & Sons Ltd. | - |
dc.title | Microaffinity purification of proteins based on photolytic elution: Toward an efficient microbead affinity chromatography on a chip | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Jong Ho | - |
dc.identifier.doi | 10.1002/elps.200410005 | - |
dc.identifier.scopusid | 2-s2.0-14944375203 | - |
dc.identifier.wosid | 000227137300026 | - |
dc.identifier.bibliographicCitation | Electrophoresis, v.26, no.3, pp.694 - 702 | - |
dc.relation.isPartOf | Electrophoresis | - |
dc.citation.title | Electrophoresis | - |
dc.citation.volume | 26 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 694 | - |
dc.citation.endPage | 702 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | SOLID-PHASE EXTRACTION | - |
dc.subject.keywordPlus | IMMUNOAFFINITY CAPILLARY-ELECTROPHORESIS | - |
dc.subject.keywordPlus | PROTEOMIC SAMPLE PREPARATION | - |
dc.subject.keywordPlus | TOTAL ANALYSIS SYSTEMS | - |
dc.subject.keywordPlus | HEPATITIS-C VIRUS | - |
dc.subject.keywordPlus | ISOTACHOPHORETIC ELECTRODESORPTION | - |
dc.subject.keywordPlus | MICROFLUIDIC DEVICES | - |
dc.subject.keywordPlus | POROUS POLYMER | - |
dc.subject.keywordPlus | BEADS | - |
dc.subject.keywordPlus | MICROEXTRACTION | - |
dc.subject.keywordAuthor | microaffinity purification | - |
dc.subject.keywordAuthor | microbead affinity chromatography | - |
dc.subject.keywordAuthor | miniaturization | - |
dc.subject.keywordAuthor | photo-cleavable ligand | - |
dc.subject.keywordAuthor | photolytic elution | - |
dc.identifier.url | https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/elps.200410005 | - |
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