Inhibition of cell cycle progression and induction of apoptosis in HeLa cells by HY558-1, a novel CDK inhibitor isolated from Penicillium minioluteum F558
DC Field | Value | Language |
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dc.contributor.author | Lim, Haeyoung | - |
dc.contributor.author | Kim, Min Kyoung | - |
dc.contributor.author | Cho, Youl-Hee | - |
dc.contributor.author | Kim, Jung Mogg | - |
dc.contributor.author | Lim, Yoongho | - |
dc.contributor.author | Lee, Chul-Hoon | - |
dc.date.accessioned | 2021-06-24T00:39:11Z | - |
dc.date.available | 2021-06-24T00:39:11Z | - |
dc.date.issued | 2004-10 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.issn | 1738-8872 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/46555 | - |
dc.description.abstract | In the course of screening for a novel inhibitor of CDC2, HY558-1 was isolated from a culture broth of Penicillium minioluteum F558. Moreover, it was found that HY558-1 had an effect on both the cell cycle regulation and apoptosis of human cervical adenocarcinoma HeLa cells. A flow cytometric analysis of HeLa cells revealed appreciable cell cycle arrest at the G1 and G2/M phases following treatment with HY558-1. Furthermore, DNA fragmentation due to apoptosis was observed in HeLa cells treated with HY558-1. To obtain further information on the cell cycle arrest and apoptotic induction induced by HY558-1, the expression of certain cell cycle and apoptosis-associated proteins was examined using a Western blot analysis. The results revealed that HY558-1 inhibited the phosphorylation of pRb and decreased the expression levels of CDK2, CDC2, and cyclin A in the cell cycle progression. It was also shown that the level of p21(WAF1/CIP1) was increased in HeLa cells treated with 0.52 mM of HY558-1. Accordingly, HY558-1 was found to inhibit the proliferation of HeLa cells through the induction of G I phase arrest by inhibiting pRb phosphorylation via an upregulation of p21(WAP1/C1P1), and G2/M phase arrest by directly inhibiting CDC2 and cyclin A. Moreover, HeLa cells treated with 0.52 mM of HY558-1 exhibited apoptotic induction associated with the cleavage of Bid and release of cytochrome c from mitochondria into the cytosol. Subsequent investigation of the activation of caspase-3 and cleavage of poly (ADP-ribose) polymerase (PARP) suggested that the mitochondrial pathway was primarily involved in the HY558-1-induced apoptosis in HeLa cells. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | 한국미생물·생명공학회 | - |
dc.title | Inhibition of cell cycle progression and induction of apoptosis in HeLa cells by HY558-1, a novel CDK inhibitor isolated from Penicillium minioluteum F558 | - |
dc.type | Article | - |
dc.publisher.location | 대한민국 | - |
dc.identifier.scopusid | 2-s2.0-8644268986 | - |
dc.identifier.wosid | 000224817300014 | - |
dc.identifier.bibliographicCitation | Journal of Microbiology and Biotechnology, v.14, no.5, pp 978 - 984 | - |
dc.citation.title | Journal of Microbiology and Biotechnology | - |
dc.citation.volume | 14 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 978 | - |
dc.citation.endPage | 984 | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART000944277 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | DEPENDENT KINASE INHIBITOR | - |
dc.subject.keywordPlus | CYTOCHROME-C RELEASE | - |
dc.subject.keywordPlus | LEUKEMIA HL-60 CELLS | - |
dc.subject.keywordPlus | RETINOBLASTOMA PROTEIN | - |
dc.subject.keywordPlus | CASPASE-8 ACTIVATION | - |
dc.subject.keywordPlus | POTENTIAL MEDIATOR | - |
dc.subject.keywordPlus | DEATH RECEPTORS | - |
dc.subject.keywordPlus | CANCER CELLS | - |
dc.subject.keywordPlus | BID CLEAVAGE | - |
dc.subject.keywordPlus | MITOCHONDRIA | - |
dc.subject.keywordAuthor | HY558-1 | - |
dc.subject.keywordAuthor | Penicillium minioluteum F558 | - |
dc.subject.keywordAuthor | cell cycle arrest | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.identifier.url | https://www.koreascience.or.kr/article/JAKO200411923003487.page | - |
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