HPLC method for simultaneous determination of cefprozil diastereomers in human plasma
- Authors
- Tae-Hwan Park; Jin-Ki Kim; Jun-Pil Jee; Jeong-Sook Park; Chong-Kook Kim
- Issue Date
- Sep-2004
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- cefprozil; stereoisomer; cis; trans; HPLC; pharmacokinetic studies in human
- Citation
- JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, v.36, no.1, pp.243 - 248
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
- Volume
- 36
- Number
- 1
- Start Page
- 243
- End Page
- 248
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/46557
- DOI
- 10.1016/j.jpba.2004.06.001
- ISSN
- 0731-7085
- Abstract
- A high-performance liquid chromatography method was developed for the determination of cefprozil diastereomers in human plasma. Cefprozil exists as cis and trans isomer at the ratio of 90:10. Plasma samples were prepared by protein precipitation using acetonitrile, trichloroacetic acid and methylene chloride. After the mixtures were vortexed and centrifuged, the aqueous supernatant was injected into a reversed-phase C-8 column. The mobile phase consisted of acetonitrile, glacial acetic acid and distilled water at the volume ratio of 5.5:1.75:92.75 (pH 2.7). The signals were monitored with UV detection at 280 nm. The calibration curves of cis and trans isomer were linear in concentration ranges of 0.1-25 and 0.02-2.5 mug/mL with the correlation coefficient of 0.9999 and 0.9989, respectively. After oral administration of cefprozil in humans, C-max and T-max of total cefprozil were 18.80 +/- 2.14 mug/mL and 2.06 +/- 0.62 h. This method was sensitive with excellent selectivity and reproducibility, and successfully applied to a bioavailability study of cefprozil in healthy subjects. (C) 2004 Elsevier B.V. All rights reserved.
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