Polyethylenimine-mediated cellular uptake, nucleus trafficking and expression of cytokine plasmid DNA
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Oh, Yukyoung | - |
dc.contributor.author | Suh, Dongchul | - |
dc.contributor.author | Kim, Jung Mogg | - |
dc.contributor.author | Choi, Hangon | - |
dc.contributor.author | Shin, K | - |
dc.contributor.author | Ko, Jaejung | - |
dc.date.accessioned | 2021-06-24T00:45:49Z | - |
dc.date.available | 2021-06-24T00:45:49Z | - |
dc.date.issued | 2002-12 | - |
dc.identifier.issn | 0969-7128 | - |
dc.identifier.issn | 1476-5462 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/46769 | - |
dc.description.abstract | Although polyethylenimine (PEI) has been widely used as a nonviral vector, there is little mechanistic understanding on PEI-mediated delivery. Here, we studied whether the expression of murine interleukin-2 (mIL-2) plasmids could be Improved by complexation with PEI at various N/P ratios, and whether the cellular uptake, nuclear translocation, and retention of plasmids could be affected by the N/P ratios. Compared with the naked mIL-2, PEI/mIL-2 complexes showed at least two orders of magnitude higher expression at Raw264 cells in the N/P ratio-dependent manner. PEI-mediated cellular uptake and nuclear trafficking of plasmids, quantitated by competitive polymerase chain reaction, also depended on the N/P ratios showing the highest cell and nuclear levels of plasmids at 10/1. The higher cellular levels of plasmid DNA after PEI-mediated delivery were also observed in other cell lines. Unlike naked plasmids, PEI/mIL2 complexes (N/P ratios greater than or equal to 4/1) showed prolonged cellular and nuclear retention of mIL-2 plasmids. The nuclear translocation and higher cellular level of plasmids given in PEI complexes were similarly observed by fluorescence microscopy. Moreover, PEI/mIL-2 complexes revealed high stability against DNase I, partly explaining the prolonged subcellular retention. These results indicate that the expression of plasmid mIL-2 might be highly enhanced by complexation with PEI and that such increased expression could be attributed by the higher cellular uptake, nuclear translocation and prolonged retention. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Nature Publishing Group | - |
dc.title | Polyethylenimine-mediated cellular uptake, nucleus trafficking and expression of cytokine plasmid DNA | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1038/sj.gt.3301735 | - |
dc.identifier.scopusid | 2-s2.0-18744389736 | - |
dc.identifier.wosid | 000179300800008 | - |
dc.identifier.bibliographicCitation | Gene Therapy, v.9, no.23, pp 1627 - 1632 | - |
dc.citation.title | Gene Therapy | - |
dc.citation.volume | 9 | - |
dc.citation.number | 23 | - |
dc.citation.startPage | 1627 | - |
dc.citation.endPage | 1632 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Genetics & Heredity | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Genetics & Heredity | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.subject.keywordPlus | GENE-TRANSFER | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | COMPLEXES | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | HEPATOCYTES | - |
dc.subject.keywordPlus | ENDOCYTOSIS | - |
dc.subject.keywordPlus | PARTICLES | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | BARRIER | - |
dc.subject.keywordAuthor | polyethylenimine | - |
dc.subject.keywordAuthor | uptake | - |
dc.subject.keywordAuthor | nucleus trafficking | - |
dc.subject.keywordAuthor | cytokine | - |
dc.subject.keywordAuthor | plasmid DNA | - |
dc.identifier.url | https://www.nature.com/articles/3301735 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
55 Hanyangdeahak-ro, Sangnok-gu, Ansan, Gyeonggi-do, 15588, Korea+82-31-400-4269 sweetbrain@hanyang.ac.kr
COPYRIGHT © 2021 HANYANG UNIVERSITY. ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.