High-Level Production of High-Purity Human and Murine Recombinant Prion Proteins Functionally Compatible to In Vitro Seeding Assay
DC Field | Value | Language |
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dc.contributor.author | Hwang, Hae-Gwang | - |
dc.contributor.author | Kim, Dae-Hwan | - |
dc.contributor.author | Lee, Jeongmin | - |
dc.contributor.author | Mo, Youngwon | - |
dc.contributor.author | Lee, Se-Hoon | - |
dc.contributor.author | Lee, Yongjin | - |
dc.contributor.author | Hyeon, Jae Wook | - |
dc.contributor.author | Lee, Sol Moe | - |
dc.contributor.author | Cheon, Yong-Pil | - |
dc.contributor.author | Choi, Eun-Kyoung | - |
dc.contributor.author | Kim, Su Yeon | - |
dc.contributor.author | Lee, Yeong Seon | - |
dc.contributor.author | Son, Young-Jin | - |
dc.contributor.author | Ryou, Chongsuk | - |
dc.date.accessioned | 2021-06-22T11:23:23Z | - |
dc.date.available | 2021-06-22T11:23:23Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2018-10 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/5299 | - |
dc.description.abstract | Recombinant (rec) prion protein (PrP) is an extremely useful resource for studying protein misfolding and subsequent protein aggregation events. Here, we report mass production of high-purity rec-polypeptide encoding the C-terminal globular domain of PrP; (90-230) for human and (89-231) for murine PrP. These proteins were expressed as His-tagged fusion proteins in E. coli cultured by a high cell-density aerobic fermentation method. RecPrPs recovered from inclusion bodies were slowly refolded under reducing conditions. Purification was performed by a sequence of metal-affinity, cation-exchange, and reverse-phase chromatography. The current procedure yielded several dozens of milligrams of recPrP per liter with >95% purity. The purified recPrPs predominantly adopted an alpha-helix-rich conformation and were functionally sufficient as substrates to measure the seeding activity of human and animal prions. Establishment of a procedure for high-level production of high-purity recPrP supports the advancement of in vitro investigations of PrP including diagnosis for prion diseases. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | 한국미생물·생명공학회 | - |
dc.title | High-Level Production of High-Purity Human and Murine Recombinant Prion Proteins Functionally Compatible to In Vitro Seeding Assay | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Ryou, Chongsuk | - |
dc.identifier.doi | 10.4014/jmb.1805.05067 | - |
dc.identifier.scopusid | 2-s2.0-85056624863 | - |
dc.identifier.wosid | 000448398800019 | - |
dc.identifier.bibliographicCitation | Journal of Microbiology and Biotechnology, v.28, no.10, pp.1749 - 1759 | - |
dc.relation.isPartOf | Journal of Microbiology and Biotechnology | - |
dc.citation.title | Journal of Microbiology and Biotechnology | - |
dc.citation.volume | 28 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 1749 | - |
dc.citation.endPage | 1759 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002398071 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | MONOCLONAL-ANTIBODIES | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PRP | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | SCRAPIE | - |
dc.subject.keywordPlus | CONVERSION | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | CONFORMATION | - |
dc.subject.keywordPlus | ISOFORM | - |
dc.subject.keywordAuthor | Expression | - |
dc.subject.keywordAuthor | high cell-density culture | - |
dc.subject.keywordAuthor | recombinant prion protein | - |
dc.subject.keywordAuthor | purification | - |
dc.subject.keywordAuthor | seeding activity | - |
dc.identifier.url | https://www.jmb.or.kr/journal/view.html?doi=10.4014/jmb.1805.05067 | - |
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