Cell-based electrochemical cytosensor for rapid and sensitive evaluation of the anticancer effects of saponin on human malignant melanoma cells
DC Field | Value | Language |
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dc.contributor.author | Hur, Won | - |
dc.contributor.author | Son, Seong-eun | - |
dc.contributor.author | Kim, Seongnyeon | - |
dc.contributor.author | Seong, Gi-hoon | - |
dc.date.accessioned | 2021-06-22T04:43:22Z | - |
dc.date.available | 2021-06-22T04:43:22Z | - |
dc.date.issued | 2021-08 | - |
dc.identifier.issn | 1567-5394 | - |
dc.identifier.issn | 1878-562X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/614 | - |
dc.description.abstract | Discovering new anticancer agents and analyzing their activities is a vital part of drug development, but it requires a huge amount of time and resources, leading to the increasing demands for more-effective techniques. Herein, a novel and simple cell-based electrochemical biosensor, referred to as a cytosensor, was proposed to investigate the electrochemical behavior of human skin malignant melanoma (SK-MEL28) cells and the anticancer effect of saponin on cell viability. To enhance both electrocatalytic properties and biocompatibility, gold nanoparticles were electrochemically deposited onto a conductive substrate, and poly-L-lysine was further added to the electrode surface. Electric signals from SK-MEL28 cells on the electrodes were obtained from cyclic voltammetry and differential pulse voltammetry. The cathodic peak current was proportional to the cell viability and showed a detection range of 2,880–40,000 cells per device with an excellent linear cell number-intensity relationship (R2= 0.9952). Furthermore, the anticancer effect of saponin on SK-MEL28 cells was clearly established at concentrations higher than 20 μM, which was highly consistent with conventional assays. Moreover, the developed electrochemical cytosensor for evaluating anticancer effects enabled rapid (<2 min), sensitive (LOQ: 2,880 cells/device), and non-invasive measurements, thus providing a new avenue for assessing the anticancer drugs in vitro. © 2021 Elsevier B.V. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Elsevier B.V. | - |
dc.title | Cell-based electrochemical cytosensor for rapid and sensitive evaluation of the anticancer effects of saponin on human malignant melanoma cells | - |
dc.type | Article | - |
dc.publisher.location | 스위스 | - |
dc.identifier.doi | 10.1016/j.bioelechem.2021.107813 | - |
dc.identifier.scopusid | 2-s2.0-85104048694 | - |
dc.identifier.wosid | 000663599500008 | - |
dc.identifier.bibliographicCitation | Bioelectrochemistry, v.140, pp 1 - 9 | - |
dc.citation.title | Bioelectrochemistry | - |
dc.citation.volume | 140 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 9 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Life Sciences & Biomedicine - Other Topics | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.subject.keywordPlus | Amino acids | - |
dc.subject.keywordPlus | Behavioral research | - |
dc.subject.keywordPlus | Biocompatibility | - |
dc.subject.keywordPlus | Cyclic voltammetry | - |
dc.subject.keywordPlus | Dermatology | - |
dc.subject.keywordPlus | Drug products | - |
dc.subject.keywordPlus | Gold deposits | - |
dc.subject.keywordPlus | Gold nanoparticles | - |
dc.subject.keywordPlus | Oncology | - |
dc.subject.keywordPlus | Anti-cancer agents | - |
dc.subject.keywordPlus | Anticancer effects | - |
dc.subject.keywordPlus | Conductive substrates | - |
dc.subject.keywordPlus | Differential pulse voltammetry | - |
dc.subject.keywordPlus | Electrocatalytic properties | - |
dc.subject.keywordPlus | Electrochemical behaviors | - |
dc.subject.keywordPlus | Electrochemical biosensor | - |
dc.subject.keywordPlus | Non- invasive measurements | - |
dc.subject.keywordPlus | Electrodes | - |
dc.subject.keywordAuthor | Anticancer drug assessment | - |
dc.subject.keywordAuthor | Cell-based biosensor | - |
dc.subject.keywordAuthor | Cytosensor | - |
dc.subject.keywordAuthor | Electrochemical detection | - |
dc.subject.keywordAuthor | Human skin malignant melanoma | - |
dc.subject.keywordAuthor | Saponin | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S1567539421000761?via%3Dihub | - |
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