Shielding effect of a PEG molecule of a mono-PEGylated peptide varies with PEG chain length
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ngoc-Thanh Thi Nguyen | - |
dc.contributor.author | Yun, Soi | - |
dc.contributor.author | Lim, Dong Woo | - |
dc.contributor.author | Lee, E. K. | - |
dc.date.accessioned | 2021-06-22T13:04:20Z | - |
dc.date.available | 2021-06-22T13:04:20Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2018-07 | - |
dc.identifier.issn | 1082-6068 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/8032 | - |
dc.description.abstract | Shielding' effect of a conjugated PEG molecule could cause a change in the electrostatic interaction characteristics of a PEGylate. We investigated how PEG chain length (or molecular weight) alters the electrostatic interaction potential of exenatide variants using their mono-PEGylates in a branched and linear form as model PEGylates. First, we performed the experiments to demonstrate the elution time changes of the mono-PEGylates conjugated with various MW PEGs (5, 10, 20, and 40kD) using cation exchange chromatography (HiTrap((R)) SP) at various pHs (2.5, 3.0, 3.5, and 4.0). Then, we calculated the net surface charge of each mono-PEGylate to propose the PEG molecule's shielding range in terms of the number of amino acids adjacent to the conjugation residue, assuming that a PEG molecule in solution sweeps out a spherical space and an exenatide molecule have a secondary structure. The net charge calculation result was well-correlated with the experimental elution time data, where 5, 10, 20, and 40kD PEG hindered the electrostatic potential of 5, 8, 12, and 17 amino acid residues in maximum, respectively, on each side of the conjugation point. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | TAYLOR & FRANCIS INC | - |
dc.title | Shielding effect of a PEG molecule of a mono-PEGylated peptide varies with PEG chain length | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lim, Dong Woo | - |
dc.identifier.doi | 10.1080/10826068.2018.1466157 | - |
dc.identifier.scopusid | 2-s2.0-85048877512 | - |
dc.identifier.wosid | 000451222800009 | - |
dc.identifier.bibliographicCitation | PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, v.48, no.6, pp.522 - 527 | - |
dc.relation.isPartOf | PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY | - |
dc.citation.title | PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY | - |
dc.citation.volume | 48 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 522 | - |
dc.citation.endPage | 527 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | INTERACTION CHROMATOGRAPHY | - |
dc.subject.keywordPlus | INTERFERON ALPHA-2A | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | PERFORMANCE | - |
dc.subject.keywordPlus | SEPARATION | - |
dc.subject.keywordPlus | FORM | - |
dc.subject.keywordAuthor | Cation exchange chromatography | - |
dc.subject.keywordAuthor | mono-PEGylate | - |
dc.subject.keywordAuthor | shielding effect | - |
dc.subject.keywordAuthor | PEG chain length | - |
dc.subject.keywordAuthor | PEG hydrodynamic radius | - |
dc.subject.keywordAuthor | PEGylation | - |
dc.identifier.url | https://www.tandfonline.com/doi/full/10.1080/10826068.2018.1466157 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
55 Hanyangdeahak-ro, Sangnok-gu, Ansan, Gyeonggi-do, 15588, Korea+82-31-400-4269 sweetbrain@hanyang.ac.kr
COPYRIGHT © 2021 HANYANG UNIVERSITY. ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.