Pre-elafin is Involved in Ultraviolet-induced Keratinocyte Apoptosis via Pro-caspase-3 Activation Associated with Cystatin-A Downregulation
DC Field | Value | Language |
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dc.contributor.author | Kim, Hyangmi | - |
dc.contributor.author | Noh, Minsoo | - |
dc.contributor.author | Bae, Ok-Nam | - |
dc.contributor.author | Lee, Chang-Hoon | - |
dc.contributor.author | Lee, Ai-Young | - |
dc.date.accessioned | 2021-06-22T14:04:23Z | - |
dc.date.available | 2021-06-22T14:04:23Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2017-05 | - |
dc.identifier.issn | 0001-5555 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/9641 | - |
dc.description.abstract | Pre-elafin controls keratinocyte integrity via cornified envelope formation and inhibition of desquamation, but its role in ultraviolet (UV)-induced keratinocyte apoptosis is unknown. This study examined the role of pre-elafin in volunteer skin samples and primary cultured normal human keratinocytes irradiated with phototoxic doses of UVA/narrow-band UVB, and in keratinocytes with pre-elafin overexpression/knockdown, under conditions of low and high calcium. Phototoxic doses of UV increased pre-elafin mRNA and protein expression in inverse proportion to keratinocyte survival. Pre-elafin overexpression under conditions of low calcium, which, in contrast to conditions of high calcium, was localized to the cytoplasm, increased keratinocyte apoptosis, whereas knockdown inhibited UV-induced apoptosis. Pre-elafin was co-localized with, but not bound to, cleaved caspase-3. Pre-elafin reduced cystatin-A expression, which was bound to pro-caspase-3. In conclusion, UV phototoxicity-induced pre-elafin inside keratinocytes prior to cornified envelope formation could be involved in UV-induced keratinocyte apoptosis via cystatin-A downregulation resulting in pro-caspase-3 activation. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ACTA DERMATO-VENEREOLOGICA | - |
dc.title | Pre-elafin is Involved in Ultraviolet-induced Keratinocyte Apoptosis via Pro-caspase-3 Activation Associated with Cystatin-A Downregulation | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Bae, Ok-Nam | - |
dc.identifier.doi | 10.2340/00015555-2621 | - |
dc.identifier.scopusid | 2-s2.0-85019040462 | - |
dc.identifier.wosid | 000401127900004 | - |
dc.identifier.bibliographicCitation | ACTA DERMATO-VENEREOLOGICA, v.97, no.5, pp.578 - 585 | - |
dc.relation.isPartOf | ACTA DERMATO-VENEREOLOGICA | - |
dc.citation.title | ACTA DERMATO-VENEREOLOGICA | - |
dc.citation.volume | 97 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 578 | - |
dc.citation.endPage | 585 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Dermatology | - |
dc.relation.journalWebOfScienceCategory | Dermatology | - |
dc.subject.keywordPlus | CORNIFIED CELL-ENVELOPE | - |
dc.subject.keywordPlus | MOLECULAR-MECHANISMS | - |
dc.subject.keywordPlus | PSORIATIC EPIDERMIS | - |
dc.subject.keywordPlus | CYTOCHROME-C | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | INHIBITOR | - |
dc.subject.keywordPlus | PROTEASE | - |
dc.subject.keywordPlus | SKIN | - |
dc.subject.keywordPlus | TRANSGLUTAMINASE | - |
dc.subject.keywordPlus | SKALP/ELAFIN | - |
dc.subject.keywordAuthor | UV | - |
dc.subject.keywordAuthor | keratinocyte apoptosis | - |
dc.subject.keywordAuthor | pre-elafin | - |
dc.subject.keywordAuthor | cellular location | - |
dc.subject.keywordAuthor | cystatin-A | - |
dc.subject.keywordAuthor | pro-caspase-3 | - |
dc.identifier.url | https://www.medicaljournals.se/acta/content/abstract/10.2340/00015555-2621 | - |
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