Progressive slowdown/prevention of cellular senescence by CD9-targeted delivery of rapamycin using lactose-wrapped calcium carbonate nanoparticles
DC Field | Value | Language |
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dc.contributor.author | Thapa, Raj Kumar | - |
dc.contributor.author | Hanh Thuy Nguyen | - |
dc.contributor.author | Jeong, Jee-Heon | - |
dc.contributor.author | Kim, Jae Ryong | - |
dc.contributor.author | Choi, Han-Gon | - |
dc.contributor.author | Yong, Chul Soon | - |
dc.contributor.author | Kim, Jong Oh | - |
dc.date.accessioned | 2021-06-22T14:21:34Z | - |
dc.date.available | 2021-06-22T14:21:34Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2017-04 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/9987 | - |
dc.description.abstract | Cellular senescence, a state of irreversible growth arrest and altered cell function, causes aging-related diseases. Hence, treatment modalities that could target aging cells would provide a robust therapeutic avenue. Herein, for the first time, we utilized CD9 receptors (overexpressed in senescent cells) for nanoparticle targeting in addition to the inherent beta-galactosidase activity. In our study, CD9 monoclonal antibody-conjugated lactose-wrapped calcium carbonate nanoparticles loaded with rapamycin (CD9-Lac/CaCO3/Rapa) were prepared for targeted rapamycin delivery to senescent cells. The nanoparticles exhibited an appropriate particle size (similar to 130 nm) with high drug-loading capacity (similar to 20%). In vitro drug release was enhanced in the presence of beta-galactosidase suggesting potential cargo drug delivery to the senescent cells. Furthermore, CD9-Lac/CaCO3/Rapa exhibited high uptake and anti-senescence effects (reduced beta-galactosidase and p53/p21/CD9/cyclin D1 expression, reduced population doubling time, enhanced cell proliferation and migration, and prevention of cell cycle arrest) in old human dermal fibroblasts. Importantly, CD9-Lac/CaCO3/Rapa significantly improved the proliferation capability of old cells as suggested by BrdU staining along with significant reductions in senescence-associated secretory phenotypes (IL-6 and IL-1 beta) (P < 0.05). Altogether, our findings suggest the potential applicability of CD9-Lac/CaCO3/Rapa in targeted treatment of senescence. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | Progressive slowdown/prevention of cellular senescence by CD9-targeted delivery of rapamycin using lactose-wrapped calcium carbonate nanoparticles | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Choi, Han-Gon | - |
dc.identifier.doi | 10.1038/srep43299 | - |
dc.identifier.scopusid | 2-s2.0-85017235170 | - |
dc.identifier.wosid | 000398957000001 | - |
dc.identifier.bibliographicCitation | SCIENTIFIC REPORTS, v.7, pp.1 - 11 | - |
dc.relation.isPartOf | SCIENTIFIC REPORTS | - |
dc.citation.title | SCIENTIFIC REPORTS | - |
dc.citation.volume | 7 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 11 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | SECRETORY PHENOTYPE | - |
dc.subject.keywordPlus | HUMAN-CELLS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | CD9 | - |
dc.subject.keywordPlus | CYTOKINES | - |
dc.subject.keywordPlus | MOTILITY | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordAuthor | HUMAN-CELLS | - |
dc.subject.keywordAuthor | SECRETORY PHENOTYPE | - |
dc.subject.keywordAuthor | CANCER | - |
dc.subject.keywordAuthor | EXPRESSION | - |
dc.subject.keywordAuthor | FIBROBLASTS | - |
dc.subject.keywordAuthor | CD9 | - |
dc.subject.keywordAuthor | SUPPRESSION | - |
dc.subject.keywordAuthor | CYTOKINES | - |
dc.subject.keywordAuthor | MOTILITY | - |
dc.subject.keywordAuthor | CULTURE | - |
dc.identifier.url | https://www.nature.com/articles/srep43299 | - |
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