Biocatalytic role of potato starch synthase III for alpha-glucan biosynthesis in Synechocystis sp PCC6803 mutants
DC Field | Value | Language |
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dc.contributor.author | Yoo, Sang-Ho | - |
dc.contributor.author | Lee, Byung-Hoo | - |
dc.contributor.author | Li, Li | - |
dc.contributor.author | Perris, Shayani D. N. | - |
dc.contributor.author | Spalding, Martin H. | - |
dc.contributor.author | Han, Sang Yun | - |
dc.contributor.author | Jane, Jay-lin | - |
dc.date.available | 2020-02-28T07:44:13Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2015-11 | - |
dc.identifier.issn | 0141-8130 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/10006 | - |
dc.description.abstract | A potato starch synthase III (PSSIII) was expressed in the Synechocystis mutants deficient in either glycogen synthase I (M1) or II (M2) to replenish alpha-(1,4) linkage synthesizing activity, resulting in new mutants, PM1 and PM2, respectively. These mutants were applied to study the role of exogenous plant starch synthase for starch/glycogen biosynthesis mechanism established in the cyanobacteria. The remaining glycogen synthase genes in PM1 and PM2 were further disrupted to make the mutants PM12 and PM21 which contained PSSIII as the sole glycogen/starch synthase. Among wild type and mutants, there were no significant differences in the amount of alpha-glucan produced. All the mutants harboring active PSSIII produced alpha-glucans with relatively much shorter and less longer alpha-1,4 chains than wild-type glycogen, which was exactly in accordance with the increase in glycogen branching enzyme activity. In fact, alpha-glucan structure of PM1 was very similar to those of PM12 and PM21, and PM2 had more intermediate chains than M2. This result suggests PSSIII may have distributive elongation property during alpha-glucan synthesis. In conclusion, the Synechocystis as an expression model system of plant enzymes can be applied to determine the role of starch synthesizing enzymes and their association during a-glucan synthesis. (C) 2015 Elsevier B.V. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | - |
dc.subject | ADP-GLUCOSE PYROPHOSPHORYLASE | - |
dc.subject | BRANCHING ENZYME-ACTIVITY | - |
dc.subject | SP PCC 6803 | - |
dc.subject | SACCHAROMYCES-CEREVISIAE | - |
dc.subject | AMYLOPECTIN STRUCTURE | - |
dc.subject | GRANULE FORMATION | - |
dc.subject | PHOTOSYSTEM-II | - |
dc.subject | GLYCOGEN | - |
dc.subject | MAIZE | - |
dc.subject | MUTATIONS | - |
dc.title | Biocatalytic role of potato starch synthase III for alpha-glucan biosynthesis in Synechocystis sp PCC6803 mutants | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000367408300089 | - |
dc.identifier.doi | 10.1016/j.ijbiomac.2015.09.008 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, v.81, pp.710 - 717 | - |
dc.identifier.scopusid | 2-s2.0-84941269352 | - |
dc.citation.endPage | 717 | - |
dc.citation.startPage | 710 | - |
dc.citation.title | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | - |
dc.citation.volume | 81 | - |
dc.contributor.affiliatedAuthor | Lee, Byung-Hoo | - |
dc.contributor.affiliatedAuthor | Han, Sang Yun | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Potato starch synthase III | - |
dc.subject.keywordAuthor | Synechocystis sp PCC6803 | - |
dc.subject.keywordAuthor | alpha-Glucan | - |
dc.subject.keywordAuthor | Glycogen branching enzyme | - |
dc.subject.keywordAuthor | Glycogen synthase | - |
dc.subject.keywordPlus | ADP-GLUCOSE PYROPHOSPHORYLASE | - |
dc.subject.keywordPlus | BRANCHING ENZYME-ACTIVITY | - |
dc.subject.keywordPlus | SP PCC 6803 | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | AMYLOPECTIN STRUCTURE | - |
dc.subject.keywordPlus | GRANULE FORMATION | - |
dc.subject.keywordPlus | PHOTOSYSTEM-II | - |
dc.subject.keywordPlus | GLYCOGEN | - |
dc.subject.keywordPlus | MAIZE | - |
dc.subject.keywordPlus | MUTATIONS | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Polymer Science | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Polymer Science | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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