Discovery of Novel Anti-prion Compounds Using In Silico and In Vitro Approaches
- Authors
- Hyeon, Jae Wook; Choi, Jiwon; Kim, Su Yeon; Govindaraj, Rajiv Gandhi; Hwang, Kyu Jam; Lee, Yeong Seon; An, Seong Soo A.; Lee, Myung Koo; Joung, Jong Young; No, Kyoung Tai; Lee, Jeongmin
- Issue Date
- 9-Oct-2015
- Publisher
- NATURE PUBLISHING GROUP
- Citation
- SCIENTIFIC REPORTS, v.5
- Journal Title
- SCIENTIFIC REPORTS
- Volume
- 5
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/10015
- DOI
- 10.1038/srep14944
- ISSN
- 2045-2322
- Abstract
- Prion diseases are associated with the conformational conversion of the physiological form of cellular prion protein (PrPC) to the pathogenic form, PrPSc. Compounds that inhibit this process by blocking conversion to the PrPSc could provide useful anti-prion therapies. However, no suitable drugs have been identified to date. To identify novel anti-prion compounds, we developed a combined structure- and ligand-based virtual screening system in silico. Virtual screening of a 700,000-compound database, followed by cluster analysis, identified 37 compounds with strong interactions with essential hotspot PrP residues identified in a previous study of PrPC interaction with a known anti-prion compound (GN8). These compounds were tested in vitro using a multimer detection system, cell-based assays, and surface plasmon resonance. Some compounds effectively reduced PrPSc levels and one of these compounds also showed a high binding affinity for PrPC. These results provide a promising starting point for the development of anti-prion compounds.
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Collections - 바이오나노대학 > 바이오나노학과 > 1. Journal Articles
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