Signaling pathway and structural features of macrophage-activating pectic polysaccharide from Korean citrus, Cheongkyool peels
DC Field | Value | Language |
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dc.contributor.author | Park, Hye-Ryung | - |
dc.contributor.author | Lee, Sue Jung | - |
dc.contributor.author | Im, Su-Bin | - |
dc.contributor.author | Shin, Myoung-Sook | - |
dc.contributor.author | Choi, Hyuk-Jun | - |
dc.contributor.author | Park, Ho-Young | - |
dc.contributor.author | Shin, Kwang-Soon | - |
dc.date.available | 2020-02-27T02:22:21Z | - |
dc.date.created | 2020-02-04 | - |
dc.date.issued | 2019-09-15 | - |
dc.identifier.issn | 0141-8130 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/1011 | - |
dc.description.abstract | To characterize the immuno-stimulating ingredient from the Korean citrus, Cheongkyool, a crude polysaccharide (CCE-0) was isolated from the pectinase digests of Cheongkyool peels, from which the complex polysaccharide CCE-I was purified to homogeneity by gel filtration. CCE-I highly enhanced the production of IL-6, TNF-alpha, and NO in RAW 264.7 cell lines. It augmented the mRNA expression of IL-6, TNF-alpha, and iNOS in a dose-dependent manner. Moreover, CCE-I dose-dependently induced phosphorylation of MAPK5 and NF-kappa B related proteins and led to the nuclear translocation of p65. The effect of CCE-I on NO and IL-6 production was suppressed by treatment with specific antibodies for TLR2, TLR4, and scavenger receptors. Conversely, the primary structure of CCE-I that exhibited potent immunostimulatory activity was characterized by sugar composition, linkage analysis, and oligosaccharide analysis after beta-elimination. The results suggested that CCE-I may be a rhamnogalacturonan-I type, highly branched polysaccharide with short arabinan and galactan side chains. (C) 2019 Elsevier B.V. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | - |
dc.subject | NF-KAPPA-B | - |
dc.subject | CELL-WALLS | - |
dc.subject | DECTIN-1 | - |
dc.subject | RECEPTORS | - |
dc.subject | IMMUNITY | - |
dc.title | Signaling pathway and structural features of macrophage-activating pectic polysaccharide from Korean citrus, Cheongkyool peels | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000484644100071 | - |
dc.identifier.doi | 10.1016/j.ijbiomac.2019.07.012 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, v.137, pp.657 - 665 | - |
dc.identifier.scopusid | 2-s2.0-85068529484 | - |
dc.citation.endPage | 665 | - |
dc.citation.startPage | 657 | - |
dc.citation.title | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | - |
dc.citation.volume | 137 | - |
dc.contributor.affiliatedAuthor | Shin, Myoung-Sook | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Cheongkyool | - |
dc.subject.keywordAuthor | Polysaccharide | - |
dc.subject.keywordAuthor | MAPK | - |
dc.subject.keywordAuthor | NF-kappa B | - |
dc.subject.keywordAuthor | Rhamnogalacwronan-I | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | CELL-WALLS | - |
dc.subject.keywordPlus | DECTIN-1 | - |
dc.subject.keywordPlus | RECEPTORS | - |
dc.subject.keywordPlus | IMMUNITY | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Polymer Science | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Polymer Science | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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