Signaling pathway and structural features of macrophage-activating pectic polysaccharide from Korean citrus, Cheongkyool peels
- Authors
- Park, Hye-Ryung; Lee, Sue Jung; Im, Su-Bin; Shin, Myoung-Sook; Choi, Hyuk-Jun; Park, Ho-Young; Shin, Kwang-Soon
- Issue Date
- 15-Sep-2019
- Publisher
- ELSEVIER
- Keywords
- Cheongkyool; Polysaccharide; MAPK; NF-kappa B; Rhamnogalacwronan-I
- Citation
- INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, v.137, pp.657 - 665
- Journal Title
- INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
- Volume
- 137
- Start Page
- 657
- End Page
- 665
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/1011
- DOI
- 10.1016/j.ijbiomac.2019.07.012
- ISSN
- 0141-8130
- Abstract
- To characterize the immuno-stimulating ingredient from the Korean citrus, Cheongkyool, a crude polysaccharide (CCE-0) was isolated from the pectinase digests of Cheongkyool peels, from which the complex polysaccharide CCE-I was purified to homogeneity by gel filtration. CCE-I highly enhanced the production of IL-6, TNF-alpha, and NO in RAW 264.7 cell lines. It augmented the mRNA expression of IL-6, TNF-alpha, and iNOS in a dose-dependent manner. Moreover, CCE-I dose-dependently induced phosphorylation of MAPK5 and NF-kappa B related proteins and led to the nuclear translocation of p65. The effect of CCE-I on NO and IL-6 production was suppressed by treatment with specific antibodies for TLR2, TLR4, and scavenger receptors. Conversely, the primary structure of CCE-I that exhibited potent immunostimulatory activity was characterized by sugar composition, linkage analysis, and oligosaccharide analysis after beta-elimination. The results suggested that CCE-I may be a rhamnogalacturonan-I type, highly branched polysaccharide with short arabinan and galactan side chains. (C) 2019 Elsevier B.V. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 한의과대학 > 한의예과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/1011)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.