Detection of cholesterol molecules with a liquid crystal-based pH-driven sensor
- Authors
- Wei, Yibin; Jang, Chang-Hyun
- Issue Date
- Jul-2015
- Publisher
- SPRINGER
- Citation
- JOURNAL OF MATERIALS SCIENCE, v.50, no.13, pp.4741 - 4748
- Journal Title
- JOURNAL OF MATERIALS SCIENCE
- Volume
- 50
- Number
- 13
- Start Page
- 4741
- End Page
- 4748
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/10349
- DOI
- 10.1007/s10853-015-9027-8
- ISSN
- 0022-2461
- Abstract
- Determining cholesterol levels is of great importance in the detection of high risk for heart disease. Here, we developed a real-time sensing strategy for free cholesterol molecules, employing ultraviolet (UV)-treated liquid crystals (LCs). When 4-cyano-4'-pentylbiphenyl (5CB), a nematic LC material, is treated with UV light, it shows a bright-to-dark optical response to a very small change in pH from 7.0 to 7.1, which can be observed with a polarizing optical microscopy at the aqueous/LC interface. In diagnostic applications, the second most widely used enzymatic reaction between cholesterol and cholesterol oxidase is able to generate H+, which could be detected by pH-sensitive LCs. Exploiting these mechanisms, a cholesterol sensor was designed and the device performed well in a range of cholesterol concentrations from 10 to 300 mg/mL, which covers the physiologically relevant range of cholesterol in the human body (90-220 mg/mL). We also determined the limit of detection, 1 x 10(-9) mg/mL, and explored the effects of inhibitors on this sensor. The results obtained from this study may offer a simple tool for the detection of cholesterol compared with conventional methods and have potential for clinical diagnostics.
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