Peroxiredoxin 1 post-transcriptionally regulates snoRNA expression
DC Field | Value | Language |
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dc.contributor.author | Kim, Eun-Kyung | - |
dc.contributor.author | Lee, Sun Young | - |
dc.contributor.author | Kim, Yosup | - |
dc.contributor.author | Ahn, Sung-Min | - |
dc.contributor.author | Jang, Ho Hee | - |
dc.date.available | 2020-02-27T02:22:39Z | - |
dc.date.created | 2020-02-04 | - |
dc.date.issued | 2019-09 | - |
dc.identifier.issn | 0891-5849 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/1049 | - |
dc.description.abstract | Peroxiredoxin 1 (Prx1) is a member of the Prx family that detoxifies various peroxide substrates through conserved catalytic cysteine residues with the use of reducing equivalents. In addition to this well-known role of Prx1, we have previously demonstrated that Prx1 also has RNA-binding properties, but its function as an RNA-binding protein (RBP) remains unknown. To characterize the role of Prx1 as an RBP, we pulled down Prx1-RNA complexes and sequenced the target RNAs of Prx1. Through sequencing and further validation studies, we revealed that Prx1 binds to a specific subset of small nucleolar RNAs (snoRNAs) and regulates these molecules at the post-transcriptional level. We also found that active cysteine residues provide a structural and functional link between these two distinct functions of Prx1 (i.e., ROS scavenging and RNA-binding activities). Prx1 functions as a snoRNA-binding protein in its reduced state, and post-transcriptionally regulates the expression of a set of snoRNAs. However, when the active cysteine residues are oxidized, Prx1 loses its activity as a snoRNA-binding protein. This study is the first report describing the novel role of Prx1 as a post-transcriptional regulator of snoRNAs. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE INC | - |
dc.relation.isPartOf | FREE RADICAL BIOLOGY AND MEDICINE | - |
dc.subject | RNA-BINDING PROTEINS | - |
dc.subject | SMALL NUCLEOLAR RNAS | - |
dc.subject | OXIDATIVE STRESS | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | SITE CYSTEINE | - |
dc.subject | PEROXIDASE | - |
dc.subject | ACID | - |
dc.subject | INSIGHTS | - |
dc.subject | SENSOR | - |
dc.subject | PRDX1 | - |
dc.title | Peroxiredoxin 1 post-transcriptionally regulates snoRNA expression | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000483920100001 | - |
dc.identifier.doi | 10.1016/j.freeradbiomed.2019.05.030 | - |
dc.identifier.bibliographicCitation | FREE RADICAL BIOLOGY AND MEDICINE, v.141, pp.1 - 9 | - |
dc.identifier.scopusid | 2-s2.0-85066609539 | - |
dc.citation.endPage | 9 | - |
dc.citation.startPage | 1 | - |
dc.citation.title | FREE RADICAL BIOLOGY AND MEDICINE | - |
dc.citation.volume | 141 | - |
dc.contributor.affiliatedAuthor | Kim, Eun-Kyung | - |
dc.contributor.affiliatedAuthor | Kim, Yosup | - |
dc.contributor.affiliatedAuthor | Ahn, Sung-Min | - |
dc.contributor.affiliatedAuthor | Jang, Ho Hee | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Peroxiredoxin1 | - |
dc.subject.keywordAuthor | Small nucleolar RNA | - |
dc.subject.keywordAuthor | RNA-binding protein | - |
dc.subject.keywordAuthor | Protein-RNA interaction | - |
dc.subject.keywordAuthor | Post-transcriptional regulation | - |
dc.subject.keywordPlus | RNA-BINDING PROTEINS | - |
dc.subject.keywordPlus | SMALL NUCLEOLAR RNAS | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | SITE CYSTEINE | - |
dc.subject.keywordPlus | PEROXIDASE | - |
dc.subject.keywordPlus | ACID | - |
dc.subject.keywordPlus | INSIGHTS | - |
dc.subject.keywordPlus | SENSOR | - |
dc.subject.keywordPlus | PRDX1 | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Endocrinology & Metabolism | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Endocrinology & Metabolism | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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