Peroxiredoxin 1 post-transcriptionally regulates snoRNA expression
- Authors
- Kim, Eun-Kyung; Lee, Sun Young; Kim, Yosup; Ahn, Sung-Min; Jang, Ho Hee
- Issue Date
- Sep-2019
- Publisher
- ELSEVIER SCIENCE INC
- Keywords
- Peroxiredoxin1; Small nucleolar RNA; RNA-binding protein; Protein-RNA interaction; Post-transcriptional regulation
- Citation
- FREE RADICAL BIOLOGY AND MEDICINE, v.141, pp.1 - 9
- Journal Title
- FREE RADICAL BIOLOGY AND MEDICINE
- Volume
- 141
- Start Page
- 1
- End Page
- 9
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/1049
- DOI
- 10.1016/j.freeradbiomed.2019.05.030
- ISSN
- 0891-5849
- Abstract
- Peroxiredoxin 1 (Prx1) is a member of the Prx family that detoxifies various peroxide substrates through conserved catalytic cysteine residues with the use of reducing equivalents. In addition to this well-known role of Prx1, we have previously demonstrated that Prx1 also has RNA-binding properties, but its function as an RNA-binding protein (RBP) remains unknown. To characterize the role of Prx1 as an RBP, we pulled down Prx1-RNA complexes and sequenced the target RNAs of Prx1. Through sequencing and further validation studies, we revealed that Prx1 binds to a specific subset of small nucleolar RNAs (snoRNAs) and regulates these molecules at the post-transcriptional level. We also found that active cysteine residues provide a structural and functional link between these two distinct functions of Prx1 (i.e., ROS scavenging and RNA-binding activities). Prx1 functions as a snoRNA-binding protein in its reduced state, and post-transcriptionally regulates the expression of a set of snoRNAs. However, when the active cysteine residues are oxidized, Prx1 loses its activity as a snoRNA-binding protein. This study is the first report describing the novel role of Prx1 as a post-transcriptional regulator of snoRNAs.
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