Characterization and application of phages isolated from sewage for reduction of Escherichia coli O157:H7 in biofilm
DC Field | Value | Language |
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dc.contributor.author | Lee, Young-Duck | - |
dc.contributor.author | Park, Jong-Hyun | - |
dc.date.available | 2020-02-28T10:44:11Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2015-01 | - |
dc.identifier.issn | 0023-6438 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/10874 | - |
dc.description.abstract | Two phages of Escherichia coli O157:H7 from a sewage sample were isolated and characterized to control E. coli O157:H7 in foods. The isolated phages, BECP2 and BECP6, belonged to the Myoviridae and Podoviridae, respectively. Their burst sizes were 33 PFU/cell for BECP2 and 51 PFU/cell for BECP6 after a latent period of 25 min. Shiga toxin genes were not detected on either the BECP2 or BECP6 phage. The two phages were susceptible to 70% ethanol. To determine the utility of the two phage mixture for biocontrol of E. coli O157:H7, we analyzed the reduction of E. coli O157:H7 in commercial milk and in biofilms. The mixture reduced the growth of E. coli O157:H7 from 6 log CFU/mL to 1 log CFU/mL after 5-h incubation at 37 degrees C in commercial milk. The mixture was applied to E. coli O157:H7 in a biofilm formed on glass wool, microtiter plate, and stainless steel coupon. The density of E. coli O157:H7 in the biofilm reduced from OD600 1.1 to 0.6 on the plate and from 6 log CFU/coupon to 2 log CFU/coupon on the coupon. Therefore, the BECP2 and BECP6 phages might be efficient sanitizing agents for reducing E. coli O157:H7 in a biofilm environment. (C) 2014 Elsevier Ltd. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.relation.isPartOf | LWT-FOOD SCIENCE AND TECHNOLOGY | - |
dc.subject | SHIGA TOXIN 2 | - |
dc.subject | CHEMICAL INACTIVATION | - |
dc.subject | BIOCONTROL AGENTS | - |
dc.subject | WASTE-WATER | - |
dc.subject | HOST-RANGE | - |
dc.subject | BACTERIOPHAGES | - |
dc.subject | FOOD | - |
dc.subject | SUSCEPTIBILITY | - |
dc.subject | CONTAMINATION | - |
dc.subject | PREVENTION | - |
dc.title | Characterization and application of phages isolated from sewage for reduction of Escherichia coli O157:H7 in biofilm | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000345106100077 | - |
dc.identifier.doi | 10.1016/j.lwt.2014.09.017 | - |
dc.identifier.bibliographicCitation | LWT-FOOD SCIENCE AND TECHNOLOGY, v.60, no.1, pp.571 - 577 | - |
dc.identifier.scopusid | 2-s2.0-84921965980 | - |
dc.citation.endPage | 577 | - |
dc.citation.startPage | 571 | - |
dc.citation.title | LWT-FOOD SCIENCE AND TECHNOLOGY | - |
dc.citation.volume | 60 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | Park, Jong-Hyun | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | E. coli O157:H7 | - |
dc.subject.keywordAuthor | Phage | - |
dc.subject.keywordAuthor | Biocontrol | - |
dc.subject.keywordAuthor | Biofilm | - |
dc.subject.keywordPlus | SHIGA TOXIN 2 | - |
dc.subject.keywordPlus | CHEMICAL INACTIVATION | - |
dc.subject.keywordPlus | BIOCONTROL AGENTS | - |
dc.subject.keywordPlus | WASTE-WATER | - |
dc.subject.keywordPlus | HOST-RANGE | - |
dc.subject.keywordPlus | BACTERIOPHAGES | - |
dc.subject.keywordPlus | FOOD | - |
dc.subject.keywordPlus | SUSCEPTIBILITY | - |
dc.subject.keywordPlus | CONTAMINATION | - |
dc.subject.keywordPlus | PREVENTION | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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