Characterization and application of phages isolated from sewage for reduction of Escherichia coli O157:H7 in biofilm
- Authors
- Lee, Young-Duck; Park, Jong-Hyun
- Issue Date
- Jan-2015
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- E. coli O157:H7; Phage; Biocontrol; Biofilm
- Citation
- LWT-FOOD SCIENCE AND TECHNOLOGY, v.60, no.1, pp.571 - 577
- Journal Title
- LWT-FOOD SCIENCE AND TECHNOLOGY
- Volume
- 60
- Number
- 1
- Start Page
- 571
- End Page
- 577
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/10874
- DOI
- 10.1016/j.lwt.2014.09.017
- ISSN
- 0023-6438
- Abstract
- Two phages of Escherichia coli O157:H7 from a sewage sample were isolated and characterized to control E. coli O157:H7 in foods. The isolated phages, BECP2 and BECP6, belonged to the Myoviridae and Podoviridae, respectively. Their burst sizes were 33 PFU/cell for BECP2 and 51 PFU/cell for BECP6 after a latent period of 25 min. Shiga toxin genes were not detected on either the BECP2 or BECP6 phage. The two phages were susceptible to 70% ethanol. To determine the utility of the two phage mixture for biocontrol of E. coli O157:H7, we analyzed the reduction of E. coli O157:H7 in commercial milk and in biofilms. The mixture reduced the growth of E. coli O157:H7 from 6 log CFU/mL to 1 log CFU/mL after 5-h incubation at 37 degrees C in commercial milk. The mixture was applied to E. coli O157:H7 in a biofilm formed on glass wool, microtiter plate, and stainless steel coupon. The density of E. coli O157:H7 in the biofilm reduced from OD600 1.1 to 0.6 on the plate and from 6 log CFU/coupon to 2 log CFU/coupon on the coupon. Therefore, the BECP2 and BECP6 phages might be efficient sanitizing agents for reducing E. coli O157:H7 in a biofilm environment. (C) 2014 Elsevier Ltd. All rights reserved.
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