Evaluation of the iNtRON VRE vanA/vanB Real-Time PCR Assay for Detection of Vancomycin-Resistant Enterococci
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Huh, Hee Jae | - |
dc.contributor.author | Jang, Mi-Ae | - |
dc.contributor.author | Seo, Ja Young | - |
dc.contributor.author | Kim, Ji-Youn | - |
dc.contributor.author | Ki, Chang-Seok | - |
dc.contributor.author | Kim, Jong-Won | - |
dc.contributor.author | Lee, Nam Yong | - |
dc.date.available | 2020-02-28T10:44:48Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2015-01 | - |
dc.identifier.issn | 2234-3806 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/10907 | - |
dc.description.abstract | Background: Recently, the iNtRON VRE vanA/vanB real-time PCR (iNtRON; iNtRON Biotechnology, Korea) assay, a multiplex real-time PCR method, was introduced. In this prospective study, we compared the iNtRON assay with the Seep lex VRE ACE detection kit (Seep lex; Seegene, Korea), a conventional multiplex PCR assay. Methods: A chromogenic agar-based culture, in which pre-selected vancomycin-resistant enterococci (VRE) was grown and subsequently plated on blood agar with vancomycin disks, was regarded as the reference method. A total of 304 consecutive rectal swab specimens were tested for VRE by culture and by iNtRON and Seep lex PCR assays. For the PCR assays, specimens were enriched for 16-24 hr before PCR. Results: VRE were isolated from 44 (14.5%) specimens by chromogenic agar-based culture. The clinical sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of the iNtRON assay were 100% (95% confidence interval: 89.8%-100%), 99.2% (96.9%-99.9%), 95.6% (83.6%-99.2%), and 100% (98.2%-100%), respectively, while those of the Seeplex assay were 97.7% (86.2%-99.9%), 99.6% (97.5%-99.9%), 97.7% (86.2%-99.9%), and 99.6% (97.5%-99.9%), respectively. The iNtRON assay had a detection limit of 3,159 copies/mu L and 13,702 copies/mu L for the vanA and vanB genes, respectively. No cross-reactivity was observed in 11 non-VRE bacterial culture isolates. Conclusions: The overall performance of the iNtRON assay was comparable to that of a chromogenic agar-based culture method for prompt identification of VRE-colonized patients in hospitals. This assay could be an alternative or supportive method for the effective control of nosocomial VRE infection. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC LABORATORY MEDICINE | - |
dc.relation.isPartOf | ANNALS OF LABORATORY MEDICINE | - |
dc.subject | VANR ASSAY | - |
dc.subject | FAECIUM | - |
dc.subject | IDENTIFICATION | - |
dc.subject | COLONIZATION | - |
dc.subject | GENE | - |
dc.subject | SURVEILLANCE | - |
dc.subject | CULTURE | - |
dc.title | Evaluation of the iNtRON VRE vanA/vanB Real-Time PCR Assay for Detection of Vancomycin-Resistant Enterococci | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000347745100011 | - |
dc.identifier.doi | 10.3343/alm.2015.35.1.76 | - |
dc.identifier.bibliographicCitation | ANNALS OF LABORATORY MEDICINE, v.35, no.1, pp.76 - 81 | - |
dc.identifier.kciid | ART001945617 | - |
dc.identifier.scopusid | 2-s2.0-84920545177 | - |
dc.citation.endPage | 81 | - |
dc.citation.startPage | 76 | - |
dc.citation.title | ANNALS OF LABORATORY MEDICINE | - |
dc.citation.volume | 35 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | Seo, Ja Young | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Real-time PCR | - |
dc.subject.keywordAuthor | Performance | - |
dc.subject.keywordAuthor | vanA | - |
dc.subject.keywordAuthor | vanB | - |
dc.subject.keywordAuthor | Vancomycin-resistant enterococci | - |
dc.subject.keywordPlus | VANR ASSAY | - |
dc.subject.keywordPlus | FAECIUM | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | COLONIZATION | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | SURVEILLANCE | - |
dc.subject.keywordPlus | CULTURE | - |
dc.relation.journalResearchArea | Medical Laboratory Technology | - |
dc.relation.journalWebOfScienceCategory | Medical Laboratory Technology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.