Evaluation of the iNtRON VRE vanA/vanB Real-Time PCR Assay for Detection of Vancomycin-Resistant Enterococci
- Authors
- Huh, Hee Jae; Jang, Mi-Ae; Seo, Ja Young; Kim, Ji-Youn; Ki, Chang-Seok; Kim, Jong-Won; Lee, Nam Yong
- Issue Date
- Jan-2015
- Publisher
- KOREAN SOC LABORATORY MEDICINE
- Keywords
- Real-time PCR; Performance; vanA; vanB; Vancomycin-resistant enterococci
- Citation
- ANNALS OF LABORATORY MEDICINE, v.35, no.1, pp.76 - 81
- Journal Title
- ANNALS OF LABORATORY MEDICINE
- Volume
- 35
- Number
- 1
- Start Page
- 76
- End Page
- 81
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/10907
- DOI
- 10.3343/alm.2015.35.1.76
- ISSN
- 2234-3806
- Abstract
- Background: Recently, the iNtRON VRE vanA/vanB real-time PCR (iNtRON; iNtRON Biotechnology, Korea) assay, a multiplex real-time PCR method, was introduced. In this prospective study, we compared the iNtRON assay with the Seep lex VRE ACE detection kit (Seep lex; Seegene, Korea), a conventional multiplex PCR assay. Methods: A chromogenic agar-based culture, in which pre-selected vancomycin-resistant enterococci (VRE) was grown and subsequently plated on blood agar with vancomycin disks, was regarded as the reference method. A total of 304 consecutive rectal swab specimens were tested for VRE by culture and by iNtRON and Seep lex PCR assays. For the PCR assays, specimens were enriched for 16-24 hr before PCR. Results: VRE were isolated from 44 (14.5%) specimens by chromogenic agar-based culture. The clinical sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of the iNtRON assay were 100% (95% confidence interval: 89.8%-100%), 99.2% (96.9%-99.9%), 95.6% (83.6%-99.2%), and 100% (98.2%-100%), respectively, while those of the Seeplex assay were 97.7% (86.2%-99.9%), 99.6% (97.5%-99.9%), 97.7% (86.2%-99.9%), and 99.6% (97.5%-99.9%), respectively. The iNtRON assay had a detection limit of 3,159 copies/mu L and 13,702 copies/mu L for the vanA and vanB genes, respectively. No cross-reactivity was observed in 11 non-VRE bacterial culture isolates. Conclusions: The overall performance of the iNtRON assay was comparable to that of a chromogenic agar-based culture method for prompt identification of VRE-colonized patients in hospitals. This assay could be an alternative or supportive method for the effective control of nosocomial VRE infection.
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