Molecular Identification of Reynoutria japonica Houtt. and R. sachalinensis (F. Schmidt) Nakai Using SNP Sites
- Authors
- 박하나; 윤창영; 김진숙; 김주환
- Issue Date
- Dec-2015
- Publisher
- 한국자원식물학회
- Keywords
- Molecular identification; Reynoutria japonica; Reynoutria sachalinensis; SNP
- Citation
- 한국자원식물학회지, v.28, no.6, pp.743 - 751
- Journal Title
- 한국자원식물학회지
- Volume
- 28
- Number
- 6
- Start Page
- 743
- End Page
- 751
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/11222
- DOI
- 10.7732/kjpr.2015.28.6.743
- ISSN
- 1226-3591
- Abstract
- Reynoutria japonica and R. sachalinensis have been used as medicinal resources in Korea. However, it is difficult to identify and determine these medicinal herbs correctly because they are usually customized and purchased as the fragmented rhizomes types. To develop molecular markers for distinguishing two species, we analyzed and compared the chloroplast DNA sequences of seven loci (atpB, matK, ccD-psaI, atpF-H, trnL-trnF, psbK-I and rpl32-trnL). Among them, we found two effective SNPs in psbK-I region for R. japonica and atpF-H region for R. sachalinensis. Based on these SNP sites, we designed the new R. japonica- specific primer which is able to amplify 300 bp fragment in psbK-I region. A similar strategy was applied for the atpF-H region of R. sachalinensis. These molecular markers would be successfully applied to recognize R. japonica and R. sachalinensis.
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