Cytotoxicity and Biological Efficacy of Exendin-4-Encapsulated Solid Lipid Nanoparticles in INS-1 Cells
- Authors
- Jun, Hee-Sook; Bae, Gongdeuk; Ko, Young Tag; Oh, Yoon Sin
- Issue Date
- Sep-2015
- Publisher
- HINDAWI LTD
- Citation
- JOURNAL OF NANOMATERIALS, v.2015
- Journal Title
- JOURNAL OF NANOMATERIALS
- Volume
- 2015
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/11857
- DOI
- 10.1155/2015/753569
- ISSN
- 1687-4110
- Abstract
- Exendin-4 (Ex-4), a peptide of glucagon-like peptide-1 receptor agonist, is a potent insulinotropic agent and alternative drug delivery systems to increase therapeutic utility have been explored. We developed exendin-4-encapsulated solid lipid nanoparticles (Eudragit Ex-4 SLNs) and compared the effects of Eudragit Ex-4 SLNs with those of native Ex-4 on INS-1 cells. We observed no significant toxic effects of nanoparticles at concentrations from 1 nM to 100 nM. Similar to Ex-4, Eudragit Ex-4 SLNs stimulated the production of cyclic AMP at 10 nM. Moreover, unlike treatment with the vehicle, treatment with 10 nM Eudragit Ex-4 SLNs increased insulin mRNA levels and insulin secretion. These insulinotropic effects of Eudragit Ex-4 SLNs were comparable to those of Ex-4. Thus, our in vitro results suggest that the biological effects of Eudragit Ex-4 SLNs are similar to those of Ex-4, and further in vivo pharmacokinetic studies are required to propose an alternative sustained release drug system.
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Collections - 약학대학 > 약학과 > 1. Journal Articles
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