A regenerative label-free fiber optic sensor using surface plasmon resonance for clinical diagnosis of fibrinogen
- Authors
- Nguyen, Tan Tai; Bea, Sun Oh; Kim, Dong Min; Yoon, Won Jung; Park, Jin-Won; An, Seong Soo A.; Ju, Heongkyu
- Issue Date
- Aug-2015
- Publisher
- DOVE MEDICAL PRESS LTD
- Keywords
- SPR; real-time assay; histidine-tagged peptide; protein sensing
- Citation
- INTERNATIONAL JOURNAL OF NANOMEDICINE, v.10, pp.155 - 163
- Journal Title
- INTERNATIONAL JOURNAL OF NANOMEDICINE
- Volume
- 10
- Start Page
- 155
- End Page
- 163
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/11972
- DOI
- 10.2147/IJN.S88963
- ISSN
- 1178-2013
- Abstract
- Purpose: We present the regenerative label-free fiber optical biosensor that exploits surface plasmon resonance for quantitative detection of fibrinogen (Fbg) extracted from human blood plasma. Materials and methods: The sensor head was made up of a multimode optical fiber with its polymer cladding replaced by metal composite of nanometer thickness made of silver, aluminum, and nickel. The Ni layer coated allowed a direct immobilization of histidine-tagged peptide (HP) on its metal surface without an additional cross-linker in between. On the coated HP layer, immunoglobulin G was then immobilized for specific capturing of Fbg. Results: We demonstrated a real-time quantitative detection of Fbg concentrations with limit of detection of similar to 10 ng/mL. The fact that the HP layer could be removed by imidazole with acid also permitted us to demonstrate the regeneration of the outermost metal surface of the sensor head for the sensor reusability. Conclusion: The sensor detection limit was estimated to be similar to 10 pM, which was believed to be sensitive enough for detecting Fbg during the clinical diagnosis of cardiovascular diseases, myocardial infarction, strokes, and Alzheimer's diseases.
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Collections - 바이오나노대학 > 나노물리학과 > 1. Journal Articles
- 바이오나노대학 > 바이오나노학과 > 1. Journal Articles
- 공과대학 > 화공생명공학과 > 1. Journal Articles
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